Dact2 Antibody - BSA Free

Images

 
Western Blot: Dact2 Antibody [NBP1-76955] - SK-N-SH cell lysate with Dact2 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: Dact2 Antibody [NBP1-76955] - Human brain tissue with Dact2 antibody at 2.5 ug/ml.
Immunofluorescence: Dact2 Antibody [NBP1-76955] - Immunofluorescence of Dact2 in Human Brain cells with Dact2 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Dact2 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human DACT2. The immunogen is located within amino acids 150 - 200 of Dact2. Amino Acid Squence: GDWRPRSVDETT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DACT2
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
Control Peptide
Dact2 Peptide (NBP1-76955PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Dact2 Antibody - BSA Free

  • bA503C24.7
  • C6orf116
  • chromosome 6 open reading frame 116
  • dapper homolog 2
  • dapper homolog 2, antagonist of beta-catenin (xenopus)
  • dapper homolog 2, antagonist of beta-catenin
  • dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis)
  • DAPPER2
  • DPR2
  • FLJ31232
  • MGC133141
  • MGC133142
  • RP11-503C24.7

Background

The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a beta-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-beta receptors. At least two isoforms of Dact2 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DACT2
Uniprot