DACH1 Antibody

Western Blot: DACH1 Antibody [NBP1-00136] - Analysis of DACH1 in Human Kidney lysate (35ug protein in RIPA buffer) using this antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DACH1 Antibody Summary

• Description: Novus Biologicals Goat DACH1 Antibody (NBP1-00136) is a polyclonal antibody validated for use in WB and ELISA. Anti-DACH1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence SPVENTPQNNECK corresponding to internal region according to NP_542937.1, NP_542938.1, NP_004383.2.
• Epitope: internal region
• Specificity: This antibody is expected to recognize all three reported isoforms (NP_542937.1; NP_542938.1; NP_004383.2).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DACH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: WB: Approx. 75 and 52 kDa band bands observed in human kidney lysates (calculated MW of 73.0 kDa band according to NP_542937.1 and of 51.9 kDa band according to NP_004383.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DACH1 Antibody

• Dach1
• DACHdachshund homolog 1
• dachshund homolog (Drosophila)
• dachshund homolog 1 (Drosophila)
• FLJ10138

Background

This gene is similar to the Drosophila melanogaster dachshund gene, which encodes a nuclear factor essential for determining cell fates in the eye, leg, and nervous system of the fly. It is a member of the Ski gene family and is thought to be involved in eye development. Four alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DACH1 Antibody (NBP1-00136)(1)

Publication using NBP1-00136

• Wu K, Yang Y, Wang C et al. DACH1 inhibits transforming growth factor-beta signaling through binding Smad4. J Biol Chem 2003-12-19 [PMID: 14525983]

Review for DACH1 Antibody (NBP1-00136) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Lin Wang IHC-Fr Mouse 09/28/2021

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: G1817

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: DACH1
• Entrez:
  • 1602(Human)
  • 13134(Mouse)
• Uniprot:
  • NP_542937.1(Human)