Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (682516)

Cytosolic Sulfotransferase 2B1/SULT2B1 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Cytosolic Sulfotransferase 2B1/SULT2B1 Monoclonal Antibody (Catalog # MAB6174) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 682516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (682516) Summary

• Immunogen: E. coli-derived recombinant human Cytosolic Sulfotransferase 2B1/SULT2B1
  Asp2-Ser365
  Accession # O00204
• Specificity: Detects human Cytosolic Sulfotransferase 2B1/SULT2B1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SULT1A1, 1A3, 1B1, 1C4, 1E1, 2A1, or 4A1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SULT2B1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (682516)

• Alcohol sulfotransferase
• Cytosolic Sulfotransferase 2B1
• EC 2.8.2
• EC 2.8.2.2
• HSST2
• HSST2sulfotransferase family cytosolic 2B member 1
• Hydroxysteroid sulfotransferase 2
• ST2B1
• Sulfotransferase 2B1
• sulfotransferase family, cytosolic, 2B, member 1
• SULT2B1

Background

SULT2B1 (Sulfotransferase 2B1; also ST2B1 and alcohol sulfotransferase) is a 45-48 kDa member of the sulfotransferase 1 family of enzymes. SULT2B1 is widely expressed, being found in platelets, stratum granulosum keratinocytes, breast and prostatic epithelium, and syncytiotrophoblast cells. SULT2B1 catalyzes the sulfonation of DHEA, a precursor for sex steroids, and cholesterol, which supports stratification of the epidermis. Human SULT2B1 is 365 amino acids (aa) in length. There are PAPS binding sites between aa 70-75 and 147-155, a myristoylation motif between aa 255-259, and a Pro-rich region between aa 305-364 that may extend enzyme half-life. There is one 43 kDa splice form (ST2B1a) that shows an eight aa substitution for aa 1-23. It is expressed in fetal brain and generates pregnenolone sulfate, a steroid that modulates neurotransmitters. Full-length human SULT2B1 shares 71% aa identity with mouse SULT2B1.        

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SULT2B1
• Uniprot:
  • O00204(Human)