Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076)

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) [NBP2-44367] - Rat Oviduct with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) [NBP2-44367] - Rat Stomach stained with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) [NBP2-44367] - Human Breast Carcinoma stained with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).

• Reactivity: Hu, Rt
• Applications: Flow, IHC, IHC-P, ICC/IF
• Clone: KRTL/1077 + KRTH/1076
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) Summary

• Immunogen: Recombinant human KRT77 and KRT76 protein
• Localization: Cytoplasmic
• Marker: Epithelial Marker
• Specificity: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
• Application Notes: Molecular weight of antigen: 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19)
  Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Reactivity Notes

Shows broad species reactivity.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT1