Cytokeratin, pan Antibody (KRT/457)

Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (KRT/457) [NBP2-45309] - Rat Oviduct stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).

Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (KRT/457) [NBP2-45309] - Human Bladder Carcinoma stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Gp, Gt, Pm
• Applications: WB, Flow, IHC, IHC-P, ICC/IF
• Clone: KRT/457
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin, pan Antibody (KRT/457) Summary

• Immunogen: Keratin-enriched preparation from cultured human epithelial cells
• Localization: Cytoplasmic
• Marker: Epithelial Marker
• Specificity: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: Multiple
  Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Reactivity Notes

Human, Monkey, Cow, Pig, Goat, Rat, Mouse, Guinea pig, Frog. Others not known.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT1
• Uniprot:
  • P13647()
  • P02538(Human)
  • P04259(Human)
  • P19013(Human)
  • P48668 (Human)