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Cytokeratin, pan Antibody (7H8C4)

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Western Blot: pan Cytokeratin Antibody (7H8C4) [NBP1-51537] - Western blot analysis using CK mouse mAb against truncated CK5 recombinant protein.
Western Blot: pan Cytokeratin Antibody (7H8C4) [NBP1-51537] - Western blot analysis using CK mouse mAb against truncated CK5 recombinant protein.
Immunocytochemistry/ Immunofluorescence: pan Cytokeratin Antibody (7H8C4) [NBP1-51537] - Immunofluorescence staining of methanol-fixed Eca-109 (left) and HepG2 (right) cells showing cytoplasmic localization.
Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (7H8C4) [NBP1-51537] - Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A),normal hepatocyte (B), colon adenocacinoma, normal ...read more
Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (7H8C4) [NBP1-51537] - Immunohistochemical analysis of paraffin-embedded human Skin tissues using CK mouse mAb.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
7H8C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Cytokeratin, pan Antibody (7H8C4) Summary

Immunogen
Purified recombinant fragment of CK5 expressed in E. Coli.
Marker
Epithelial marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT1
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Antibody (3C2F5)
NBP2-61736
Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of esophageal cancer tissues using KRT10 mouse mAb with DAB staining.
Flow Cytometry: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, IHC, IHC-P
Cytokeratin 14
Antibody (LL002)
NBP2-34270
Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Cytokeratin 5
Antibody (2C2)
NBP2-22194
Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.
(1 Review)
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Involucrin
Antibody (SY5)
NB100-2727
Western Blot: Involucrin Antibody (SY5) [NB100-2727] - Involucrin from primary human epidermal keratinocyte lysates grown in low calcium (0.07 mM) for 5 days or differentiated with high calcium (1.5 mM) for 1-5 days. Block in 5% nonfat dry milk in TBST incubated at RT for 1 hr, antibody 1:500 in TBST, overnight at 4C. Image from verified customer review.
Immunohistochemistry-Paraffin: Involucrin Antibody (SY5) [NB100-2727] - Staining of human Tonsil with Involucrin Monoclonal Antibody (SY5).
(1 Review)
(1 Publication)
Species: Hu, Po, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Filaggrin Antibody
NBP1-87528
Immunocytochemistry/Immunofluorescence: Filaggrin Antibody [NBP1-87528] - Staining of human cell line HaCaT shows localization to vesicles.
Immunohistochemistry-Paraffin: Filaggrin Antibody [NBP1-87528] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Filaggrin Antibody [NBP1-87528] - Staining of human kidney shows low expression as expected.
(1 Review)
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Endostatin [HRP]
DNST0
(21 Publications)
Species: Hu
OXSM Antibody
NBP1-84730
Western Blot: OXSM Antibody [NBP1-84730] - Analysis in control (vector only transfected HEK293T lysate) and OXSM over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: OXSM Antibody [NBP1-84730] - Staining of human cell line A-431 shows localization to cytosol & mitochondria.
Immunohistochemistry-Paraffin: OXSM Antibody [NBP1-84730] - Staining of human cerebral cortex.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Cytokeratin 3
Antibody (KRT3/2130)
NBP2-79718
Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 3 Antibody (KRT3/2130).
Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Formalin-fixed, paraffin-embedded human Ovary stained with Cytokeratin 3 Antibody (KRT3/2130).
Protein Array: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 3 Antibody (KRT3/2130). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.
Species: Hu
Applications: IHC, IHC-P, PA
Cytokeratin 4
Antibody (SN2001)
NBP2-67727
Western Blot: Cytokeratin 4 Antibody (SN2001) [NBP2-67727] - Analysis of Cytokeratin 4 on 293T cells lysates using anti-Cytokeratin 4 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN2001) [NBP2-67727] - Staining Cytokeratin 4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (SN2001) [NBP2-67727] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 4 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Cytokeratin 13
Antibody (SN71-09)
NBP2-67689
Western Blot: Cytokeratin 13 Antibody (SN71-09) [NBP2-67689] - Analysis of Cytokeratin 13 on human lung lysates using anti-Cytokeratin 13 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Cytokeratin 13 Antibody (SN71-09) [NBP2-67689] - Staining Cytokeratin 13 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody (SN71-09) [NBP2-67689] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 13 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Fi, Ze
Applications: WB, ICC/IF, IHC, IHC-P
Cytokeratin 16
Antibody (OTI2H8)
NBP2-45538
Western Blot: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 16.
Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu
Applications: WB, IHC, IHC-P
Choline kinase
alpha Antibody
NBP1-85630
Western Blot: Choline kinase alpha Antibody [NBP1-85630] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-CHKA antibody. Corresponding CHKA RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
Choline Kinase
beta [Unconjugated]
8184-CK
Choline Kinase beta [Unconjugated]
Species: Hu
Cytokeratin 19
Antibody
NB100-687
Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.
Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep13320), licensed under a CC-BY licence.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Albumin Antibody
NB600-41532
Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.
(36 Publications)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Loricrin Antibody
NBP1-33610
Immunohistochemistry: Loricrin Antibody [NBP1-33610] - Prominent differences in the expressions of innate immune and barrier molecules between sebaceous gland poor (SGR) and sebaceous gland rich (SGP) skin regions. Representative images for immunostaining and quantification of epidermal levels of LOR in SGP and SGR skin sections. Images of negative control stainings are shown in the bottom left corner of SGP immunostainings. Size bars=100um. The graphs show the mean +/- SEM of measured protein levels (*p<0.05; **p<0.01; ***p<0.001, as determined by Mann-Whitney U-test). Image collected and cropped by CiteAb from the following publication (frontiersin.org/articles/10.3389/fimmu.2018.00424/full), licensed under a CC-BY licence.
Western Blot: Loricrin Antibody [NBP1-33610] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Loricrin Antibody [NBP1-33610] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.
(3 Reviews)
(2 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Cytokeratin 8
Antibody
NBP2-16094
Knockout Validated: Cytokeratin 8 Antibody [NBP2-16094] - Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KO
KCNN3 Antibody
NBP2-85137
Western Blot: KCNN3 Antibody [NBP2-85137] - Host: Rabbit. Target Name: KCNN3. Sample Tissue: Human THP-1 Whole Cell. Antibody Dilution: 1ug/ml
Immunohistochemistry: KCNN3 Antibody [NBP2-85137] - Sample Type: Rhesus macaque spinal cord. Primary Antibody Dilution: 1:300. Secondary Antibody: Donkey anti Rabbit 488. Secondary Antibody Dilution: 1:500. Color/Signal Descriptions: Green: KCNN3. Gene Name: KCNN3. Submitted by: Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School
Western Blot: KCNN3 Antibody [NBP2-85137] - WB Suggested Anti-KCNN3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Daudi cell lysateKCNN3 is supported by BioGPS gene expression data to be expressed in Daudi
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
Kir2.1 Antibody
(S112B-14)
NBP2-12900
Western Blot: Kir2.1 Antibody (S112B-14) [NBP2-12900] - analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112B-14. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry: Kir2.1 Antibody (S112B-14) [NBP2-12900] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P, MiAr
Cytokeratin, pan
Antibody (7H8C4)
NBP1-51537
Cytokeratin, pan Antibody (7H8C4)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

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FAQs for Cytokeratin, pan Antibody (NBP1-51537). (Showing 1 - 1 of 1 FAQ).

  1. I would like to ask a question about two antibodies of your company they are antibodies for pan cytokeratin code NBP1-51537 and NBP1-51566 which are monoclonal antibodies against human cytokeratin (clone 7H8C4 and 7H8, respectively). I would like to know if besides human they also react with other species (eg mouse)?
    • These antibodies have only been tested to react with human. If you have any further questions please feel free to let me know.

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Bioinformatics

Gene Symbol KRT1
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