Cytokeratin 8 Antibody - BSA Free

Images

 
Orthogonal Strategies: Western Blot: Cytokeratin 8 Antibody [NBP2-14177] - Analysis in human cell lines MCF-7 and SK-MEL-30 using anti-KRT8 antibody. Corresponding KRT8 RNA-seq data are presented for the same ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody [NBP2-14177] - Staining of human cell line MCF7 shows positivity in intermediate filaments.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-14177] - Staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.
Western Blot: Cytokeratin 8 Antibody [NBP2-14177] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
       

Orthogonal Strategies

 

Order Details

Cytokeratin 8 Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK
Marker
Epithelial Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Cytokeratin 8 Recombinant Protein Antigen (NBP2-14177PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 8 Antibody - BSA Free

  • CARD2
  • CK8
  • CK-8
  • CYK8
  • Cytokeratin 8
  • Cytokeratin-8
  • EndoA
  • K2C8
  • K8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • KRT8
  • Type-II keratin Kb8

Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Cytokeratin 8 Products

Research Areas for Cytokeratin 8 Antibody (NBP2-14177)

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Blogs on Cytokeratin 8.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT8