Cytokeratin 8 Antibody (SPM192)

Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SPM192) [NBP2-34352] - Immunofluorescence Analysis of HeLa cells labeling CK8 with Cytokeratin 8 Antibody (SPM192) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SPM192) [NBP2-34352] - Formalin-fixed paraffin-embedded human lung carcinoma stained with cytokeratin monoclonal antibody.

Flow Cytometry: Cytokeratin 8 Antibody (SPM192) [NBP2-34352] - An intracellular stain was performed on HeLa cells with Cytokeratin 8 antibody (SPM192) NBP2-34400AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu, Rt(-)
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: SPM192
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 8 Antibody (SPM192) Summary

• Immunogen: Keratin preparation from a human carcinoma
• Localization: Cytoplasmic
• Specificity: Epitope of this MAb is located between aa343-357. Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1.0ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 1-2ug/500ug
• Application Notes: ELISA (For coating, order NBP2-34400 (BSA free version of NBP2-34352)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 52.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cytokeratin 8 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for Cytokeratin 8 Antibody (SPM192)

• CARD2
• CK8
• CK-8
• CYK8
• Cytokeratin 8
• Cytokeratin-8
• EndoA
• K2C8
• K8
• keratin 8
• keratin, type II cytoskeletal 8
• keratin-8
• KO
• KRT8
• Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT8
• Uniprot:
  • P05787(Human)