Cytokeratin 8 Antibody (OTI1B12)

Images

 
Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 8 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 8.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Cytokeratin 8 antibody staining of mouse colon epithelium. IHC-P image submitted by a verified customer review.
Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 8 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Immunofluorescent staining of A549 cells using anti-Cytokeratin 8 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Immunofluorescent staining of HeLa cells using anti-Cytokeratin 8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI1B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Cytokeratin 8 Antibody (OTI1B12) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
Marker
Epithelial Cell Marker
Specificity
This antibody is specific for Homo sapiens keratin 8 (KRT8).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:150
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2500-5000
Theoretical MW
53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-48281 in the following applications:

Publications
Read Publication using NBP1-48281.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 8 Antibody (OTI1B12)

  • CARD2
  • CK8
  • CK-8
  • CYK8
  • Cytokeratin 8
  • Cytokeratin-8
  • EndoA
  • K2C8
  • K8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • KRT8
  • Type-II keratin Kb8

Background

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P

Publications for Cytokeratin 8 Antibody (NBP1-48281)(1)

We have publications tested in 1 application: ICC/IF.


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Review for Cytokeratin 8 Antibody (NBP1-48281) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-48281:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Cytokeratin 8 NBP1-48281
Enlarge
5
reviewed by:
Abigail Stone
Immunohistochemistry-Paraffin Mouse 11/12/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedColon epithelium
SpeciesMouse
LotOTI1B12

Comments

CommentsPrimary antibody dilution: 1:200. Secondary antibody dilution: 1:300.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 8 Antibody (NBP1-48281) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Cytokeratin 8 Antibody (NBP1-48281)

Discover related pathways, diseases and genes to Cytokeratin 8 Antibody (NBP1-48281). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Cytokeratin 8.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Abigail Stone
11/12/2021
Application: Immunohistochemistry-Paraffin
Species: Mouse

Bioinformatics

Gene Symbol KRT8
Entrez