Cytokeratin 8/18 Antibody (C-51)

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Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (C-51) [NBP2-44929] - Human Lung Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (C-51).

Product Details

Summary
Reactivity Hu, Rt, Po, Ma(-), Pm, Pm, Sh, Ch(-), Do(-), Mu(-), Rb(-), Xp(-)Species Glossary
Applications WB, Flow, IHC, IHC-P, ICC/IF
Clone
C-51
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Catalog# & Formulation Size Price

Cytokeratin 8/18 Antibody (C-51) Summary

Immunogen
Cytoskeleton preparation from HeLa cells
Localization
Cytoplasmic
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 17q21.2 (CK8); 12q13.13 (CK18)
Molecular weight of antigen: 52.5kDa (CK8); 45kDa (CK18)
Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Publications
Read Publications using
NBP2-44929 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Human, Monkey, Cow, Sheep, Pig.Rat reactivity reported in scientific literature (PMID: 28975767). Does not react with mouse, ferret, Rabbit, donkey, Chicken, xenopus laevis. Others not known.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytokeratin 8/18 Antibody (C-51)

  • CARD2
  • CK8
  • CK-8
  • CYK8cytokeratin 8
  • Cytokeratin-8
  • K2C8
  • K8cytokeratin-8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Rt, Po, Ma(-), Pm, Pm, Sh, Ch(-), Do(-), Mu(-), Rb(-), Xp(-)
Applications: WB, Flow, IHC, IHC-P, ICC/IF

Publications for Cytokeratin 8/18 Antibody (NBP2-44929)(2)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol KRT8