Cytokeratin 8/18 Antibody (C-43 + DC10)

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (C-43 + DC10) [NBP2-44925] - Human Ovarian Cancer stained with Cytokeratin 8/18 Monoclonal Antibody (C-43 + DC10).

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (C-43 + DC10) [NBP2-44925] - Human Lung Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (C-43 + DC10).

• Reactivity: Hu
• Applications: WB, Flow, IHC, IHC-P, ICC/IF
• Clone: C-43 + DC10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 8/18 Antibody (C-43 + DC10) Summary

• Immunogen: Keratin preparation from a human carcinoma (C-43); PMC-42 human breast carcinoma cells (DC10)
• Localization: Cytoplasmic
• Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT8
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
• Application Notes: Hu-chromosome location: 17q21.2 (CK8); 12q13.13 (CK18)
  Molecular weight of antigen: 52.5kDa (CK8); 45kDa (CK18)
  Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 8/18 Antibody (C-43 + DC10)

• CARD2
• CK8
• CK-8
• CYK8cytokeratin 8
• Cytokeratin-8
• K2C8
• K8cytokeratin-8
• keratin 8
• keratin, type II cytoskeletal 8
• keratin-8
• KO
• Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT8
• Uniprot:
  • P05783(Human)
  • P05787(Human)