Cytokeratin 8/18 Antibody (B22.1/B23.1) - IHC-Prediluted

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P
Clone
B22.1/B23.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

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Catalog# & Formulation Size Price

Cytokeratin 8/18 Antibody (B22.1/B23.1) - IHC-Prediluted Summary

Immunogen
Cytoskeletal preparation from HeLa epitheliod carcinoma cells (B22.1) and PMC-42 breast carcinoma cells of human origin (B23.1)
Localization
Cytoplasmic
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
Application Notes
Molecular weight of antigen: 52.5kDa (CK8); 45kDa (CK18) Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Cytokeratin 8/18 Antibody (B22.1/B23.1) - IHC-Prediluted

  • CARD2
  • CK8
  • CK-8
  • CYK8cytokeratin 8
  • Cytokeratin-8
  • K2C8
  • K8cytokeratin-8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KRT8