Cytokeratin 7 Antibody (RCK105) - BSA Free

• Reactivity: Hu, Mu, Rt, Po, Ca, Fe, Ha, Ma
• Applications: WB, Flow, ICC/IF, IHC, IHC
• Clone: RCK105
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Cytokeratin 7 Antibody (RCK105) - BSA Free Summary

• Description: Novus Biologicals Mouse Cytokeratin 7 Antibody (RCK105) - BSA Free (NB120-9021) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Cytokeratin 7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Cytokeratins from the human bladder carcinoma cell line T24.
• Localization: Cytoplasmic
• Specificity: Reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100-1:200
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100-1:200
  • Western Blot 1:100-1:1000
• Application Notes: Use in IHC-Whole mount reported in scientific literature (PMID: 28977592).
• Control:

  Cytokeratin 7 Overexpression Lysate

Reactivity Notes

Ferret reactivity reported in scientific literature (PMID: 28977592).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Cytokeratin 7 Antibody (RCK105) - BSA Free

• CK7
• CK-7
• Cytokeratin 7
• cytokeratin-7
• K2C7
• K7
• K7keratin, 55K type II cytoskeletal
• keratin 7
• keratin, type II cytoskeletal 7
• keratin-7
• MGC129731
• MGC3625
• sarcolectin
• SCLkeratin, simple epithelial type I, K7
• type II mesothelial keratin K7
• Type-II keratin Kb7

Background

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 7 Antibody (NB120-9021)(1)

Publication using NB120-9021

• Sun X, Yi Y, Xie W et al. CFTR Influences Beta Cell Function and Insulin Secretion Through Non-Cell Autonomous Exocrine-Derived Factors. Endocrinology 2017-10-01 [PMID: 28977592] (IHC-WhMt, Ferret)

Bioinformatics

• Gene Symbol: KRT7
• Entrez:
  • 477602(Canine)
  • 3855(Human)
  • 110310(Mouse)
  • 300242(Rat)