Cytokeratin 5/8 Antibody (RCK102) [Allophycocyanin]

• Reactivity: Hu, Mu, Rt, Po, Ca, Fe, Ha, Rb
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: RCK102
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Cytokeratin 5/8 Antibody (RCK102) [Allophycocyanin] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with Cytokeratins from a Human lung cancer cell line (MR21).
• Specificity: This antibody is a Cytokeratin antibody reacting with Cytokeratin 5 and Cytokeratin 8. This monoclonal antibody recognizes virtually all epithelial tissues and carcinomas.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: .

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 5/8 Antibody (RCK102) [Allophycocyanin]

• cell proliferation-inducing protein 46
• CK18
• CK5
• CYK18
• DDD
• K18
• K1CO
• K5
• Keratin 18
• Keratin 5
• Keratin
• KRT18
• krt5
• KRT5A
• KRTB
• type I cytoskeletal 18
• type II cytoskeletal 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Cytokeratin 5/8 Antibody (NBP1-97730APC). (Showing 1 - 1 of 1 FAQ).

1. I have purchased your Cytokeratin 5/8 Antibody (NBP1-97730APC) and my question is whether this will be the proper isotype control since it is the kappa light chain of IgG1 and not the entire IgG1?
   • Our cytokeratin 5/8 antibody with catalogue number NBP1-97730APC is a mouse monoclonal of the isotype IgG1. When the light chain is not listed it is likely that the light chain sub-class has not been determined, however the light chains of murine serum immunoglobulins are kappa in the majority of cases, and the following product NBP1-43319APC should be suitable for your requirements.

Bioinformatics

• Gene Symbol: KRT5