Cytokeratin 5/14 Antibody (XM26/LL002)

Immunohistochemistry-Paraffin: Cytokeratin 5/14 Antibody (XM26 & LL002) [NB110-59922] - Formalin fixed paraffin embedded human tonsil stained with Cytokeratin 5/14 (NB110-59922).

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: XM26/LL002
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cytokeratin 5/14 Antibody (XM26/LL002) Summary

• Description: This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.
• Immunogen: XM26: prokaryotic recombinant protein corresponding to 103 amino acid portion of C-terminal region of the human cytokeratin 5 molecule. LL002: Synthetic peptide of the extreme c-terminal of human keratin-14
• Localization: Cytoplasmic
• Specificity: This antibody cocktail reacts with cytokeratin 5 intermediate filament protein as well as to 50 kD cytokeratin protein designated cytokeratin 14. Basal cells of human epidermis express acidic cytokeratin 14 and basic cytokeratin 5. Cytokeratin 5 is a 58 kD protein which is closely related to cytokeratin 6. Cytokeratin 5 and 6 are expressed in basal cell epithelioma, basal cells of prostate, urothelium, vagina, squamous cell carcinomas of skin, tongue, epiglottis, and in the rectal-anal region. Cytokeratin 5 can be useful in the destinction of mesotheliomas from most adenocarcinomas. Cytokeratin 14 belongs to the type A (acidic) subfamily of low MW cytokeratins and exists in combination with cytokeratin 15. This antibody can be useful to distinguish stratified ephithelial cells from simple epithelial cells.
• Isotype: IgG1/IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:25-1:75
  • Immunohistochemistry-Paraffin 1:25-1:75
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
• Control:

  Human Skin Whole Tissue Lysate (Adult Whole Normal)
  Cytokeratin 5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 5/14 Antibody (XM26/LL002)

• 58 kDa cytokeratin
• CK5
• CK-5
• cytokeratin-5
• DDD
• EBS2
• epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
• K5
• keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
• keratin 5
• keratin, type II cytoskeletal 5
• Keratin-5
• KRT5A
• Type-II keratin Kb5

Background

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 5/14 Antibody (NB110-59922)(2)

Publications using NB110-59922

• Peramo A, Feinberg SE, Marcelo CL. Characterization of cultured epithelial cells using a novel technique not requiring enzymatic digestion for subculturing Cell Tissue Bank 2012-11-13 [PMID: 23149549] (ICC/IF, Human)

FAQs for Cytokeratin 5/14 Antibody (NB110-59922). (Showing 1 - 1 of 1 FAQs).

1. Has this antibody been tested on mouse tissues? Was there any cross-reactivity? 
   • This antibody has not been tested on mouse tissues, and is only guaranteed to work for human samples.

Bioinformatics

• Gene Symbol: KRT5
• Entrez:
  • 3852(Human)