Cytokeratin 3/2p Antibody (2Q1040(AE5)) - BSA Free

• Reactivity: Hu, Mu, Bv, Rb
• Applications: WB, IHC
• Clone: 2Q1040(AE5)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Cytokeratin 3/2p Antibody (2Q1040(AE5)) - BSA Free Summary

• Description: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Immunogen: Rabbit corneal epithelial keratin
• Specificity: Recognizes the 64kD polypeptide/keratin K3 (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT3
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Application Notes: Immunohistochemistry (Paraffin): 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete's acid-alcohol-formalin following trypsin digestion antigen retrieval.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2
• Preservative: 0.09% Sodium Azide
• Purity: Ion exchange chromatography

Notes

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Alternate Names for Cytokeratin 3/2p Antibody (2Q1040(AE5)) - BSA Free

• 65 KDa Cytokeratin
• CK3
• CK-3
• cytokeratin-3
• keratin 3
• Keratin
• Keratin, Type II Cytoskeletal 3
• Keratin-3
• Type II Cytoskeletal 3
• Type-II keratin Kb3

Background

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. The cytokeratins are expressed in combinations which characterize the type of epithelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT3
• Uniprot:
  • P12035(Human)
  • Q01546(Human)