Cytokeratin 20 Antibody (Ks20.8)

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (Ks20.8) [NB120-854] - Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 20 antibody (NB120-854).

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (Ks20.8) [NB120-854] - Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 20 antibody.

• Reactivity: Hu
• Applications: IHC
• Clone: Ks20.8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cytokeratin 20 Antibody (Ks20.8) Summary

• Description: Novus Biologicals Mouse Cytokeratin 20 Antibody (Ks20.8) (NB120-854) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Cytokeratins isolated from human micro-dissected villi of human duodenal mucosa.
• Localization: Cytoplasmic
• Specificity: This antibody reacts with cytokeratin polypeptide of 46 kDa in immunoblot analyses. Studies have shown that a marked difference exists in the expression of Cytokeratin 20 among various carcinomas.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT20
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
• Application Notes: IHC-P: recommended incubation time of 30 min at RT.
• Theoretical MW: 48.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cytokeratin 20 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Cytokeratin 20 Antibody (Ks20.8)

• CD20
• CK20
• CK-20
• cytokeratin-20
• K20cytokeratin 20
• keratin 20
• keratin, type I cytoskeletal 20
• keratin-20
• KRT21
• MGC35423
• Protein IT

Background

Cytokeratin 20 is 46 kDa intermediate filament protein that has been identified with expression primarily restricted to gastric and intestinal epithelium, urothelium, and Merkel cells. It is a unique type I keratin that is expressed in adenocarcinomas

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT20
• Entrez:
  • 54474(Human)
• Uniprot:
  • P35900(Human)