Cytokeratin 20 Antibody (CL9390) - BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932] - Analysis in human rectum and prostate tissues using NBP2-88932 antibody. Corresponding KRT20 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932] - Staining of human kidney shows no positivity in cells in tubules as expected.

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932] - Staining of human prostate shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932] - Staining of human rectum shows strong membranous / cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: IHC
• Clone: CL9390
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Cytokeratin 20 Antibody (CL9390) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P35900, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT20
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, containing 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Cytokeratin 20 Antibody (CL9390) - BSA Free

• CD20
• CK20
• CK-20
• cytokeratin-20
• K20cytokeratin 20
• keratin 20
• keratin, type I cytoskeletal 20
• keratin-20
• KRT21
• MGC35423
• Protein IT

Background

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT20