Cytokeratin 20 Antibody (2G3-1C8) - Azide and BSA Free

Western Blot: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - KRT20 monoclonal antibody (M01), clone 2G3-1C8 Analysis of KRT20 expression in A-431.

Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of monoclonal antibody to KRT20 on U-2 OS cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of monoclonal antibody to KRT20 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 3 ug/ml.

Western Blot: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody (M01), clone 2G3-1C8.Lane 1: KRT20 transfected lysate(48.5 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of monoclonal antibody to KRT20 on HeLa cell. Antibody concentration 10 ug/ml.

ELISA: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Detection limit for recombinant GST tagged KRT20 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2G3-1C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cytokeratin 20 Antibody (2G3-1C8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Cytokeratin 20 Antibody (2G3-1C8) - Azide and BSA Free (H00054474-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: KRT20 (AAH31559, 1 a.a. ~ 424 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDFSRRSFHRSLSSSLQAPVVSTVGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMAMQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI
• Specificity: KRT20 - keratin 20
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT20
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cytokeratin 20 Antibody (2G3-1C8) - Azide and BSA Free

• CD20
• CK20
• CK-20
• cytokeratin-20
• K20cytokeratin 20
• keratin 20
• keratin, type I cytoskeletal 20
• keratin-20
• KRT21
• MGC35423
• Protein IT

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT20
• Entrez:
  • 54474(Human)
• OMIM:
  • 608218(Human)
• Uniprot:
  • AAH31559(Human)