Western Blot: Cytokeratin 20 Antibody (10D8) [NBP2-42616] - The Recombinant Human KRT20 (25ng) and Cell lysates of HeLa, A431(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 20 Antibody (10D8) [NBP2-42616] - Analysis of Cytokeratin 20 in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT20 antibody ...read more
Flow Cytometry: Cytokeratin 20 Antibody (10D8) [NBP2-42616] - An intracellular stain was performed on HeLa cells with Cytokeratin 20 Antibody (10D8) NBP2-42616AF488 (blue) and a matched isotype control (orange). Cells ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4), 10% Glycerol
0.02% Sodium Azide
Protein A purified
Alternate Names for Cytokeratin 20 Antibody (10D8) - BSA Free
keratin, type I cytoskeletal 20
Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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