Cytokeratin 20 Antibody (10D8) - BSA Free

Images

 
Western Blot: Cytokeratin 20 Antibody (10D8) [NBP2-42616] - The Recombinant Human KRT20 (25ng) and Cell lysates of HeLa, A431(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 20 Antibody (10D8) [NBP2-42616] - Analysis of Cytokeratin 20 in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT20 antibody ...read more
Flow Cytometry: Cytokeratin 20 Antibody (10D8) [NBP2-42616] - An intracellular stain was performed on HeLa cells with Cytokeratin 20 Antibody (10D8) NBP2-42616AF488 (blue) and a matched isotype control (orange). Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
10D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Cytokeratin 20 Antibody (10D8) - BSA Free Summary

Immunogen
Recombinant human Cytokeratin 20 (1-424aa) purified from E.coli.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT20
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence Flow
  • Western Blot 1:3000
Publications
Read Publication using
NBP2-42616 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Cytokeratin 20 Antibody (10D8) - BSA Free

  • CD20
  • CK20
  • CK-20
  • cytokeratin-20
  • K20cytokeratin 20
  • keratin 20
  • keratin, type I cytoskeletal 20
  • keratin-20
  • KRT21
  • MGC35423
  • Protein IT

Background

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 20 Antibody (NBP2-42616)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: FLOW.


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FLOW
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT20
Uniprot