Cytokeratin 2 Antibody

Images

 
Western Blot: Cytokeratin 2 Antibody [NBP1-31423] - Sample (30 ug of whole cell lysate) A: A431
Immunocytochemistry/ Immunofluorescence: Cytokeratin 2 Antibody [NBP1-31423] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody [NBP1-31423] - Paraffin-embedded H661 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.67 mg/ml

Order Details

Cytokeratin 2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytokeratin 2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Canine (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.67 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 2 Antibody

  • CK-2e
  • cytokeratin-2e
  • epidermal ichthyosis bullosa of Siemens
  • Epithelial keratin-2e
  • K2e
  • keratin 2
  • keratin 2A (epidermal ichthyosis bullosa of Siemens)
  • keratin, type II cytoskeletal 2 epidermal
  • Keratin-2 epidermis
  • keratin-2e
  • KRT2AMGC116967
  • KRT2EMGC116968
  • KRTE
  • Type-II keratin Kb2

Background

Cytokeratins, a group of at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 4, 5, 6 and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin peptide 4 (59 kDa) is the secondary type II keratin expressed in non cornified stratified squamous epithelia. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia, while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. Cytokeratins 10, 13 and 18 are members of the type I acidic subfamily. Cytokeratin peptide 10 (56 kDa) is the secondary type I keratin expressed in cornified epithelia. Cytokeratin 13 (54 kDa) is the secondary type I keratin expressed in non-cornified stratified squamous epithelia. Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells. Cytokeratin is a heterotetramer of two type I and two type II keratins. Keratin 1 is generally associated with keratin 10. Defects in Cytokeratin are a cause of epidermolytic hyperkeratosis, also known as bullous congenital ichthyosiform erythroderma (BIE), which is a hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. Defects in Cytokeratin are also the cause of Curth Macklin type ichthyosis hystrix, nonepidermolytic palmoplantar keratoderma, annular epidermolytic ichthyosis and keratosis palmoplantaris striata III (PPKS3). PPKS3 is an autosomal dominant disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of nonpalmoplantar skin, and both hair and nails are normal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT2