Cytokeratin 2 Antibody - BSA Free

Western Blot: Cytokeratin 2 Antibody [NBP1-31423] - Sample (30 ug of whole cell lysate) A: A431

Immunocytochemistry/Immunofluorescence: Cytokeratin 2 Antibody [NBP1-31423] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody [NBP1-31423] - H661 xenograft, using KRT2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunocytochemistry/ Immunofluorescence: Cytokeratin 2 Antibody [NBP1-31423] - Cytokeratin 2 antibody detects Cytokeratin 2 protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cytokeratin 2 stained by Cytokeratin 2 antibody (NBP1-31423) diluted at 1:500.

Western Blot: Cytokeratin 2 Antibody [NBP1-31423] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 2 antibody (NBP1-31423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cytokeratin 2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Cytokeratin 2 Antibody - BSA Free (NBP1-31423) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Cytokeratin 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytokeratin 2. The exact sequence is proprietary.
• Predicted Species: Chimpanzee (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KRT2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Canine (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Cytokeratin 2 Antibody - BSA Free

• CK-2e
• Cytokeratin-2e
• epidermal ichthyosis bullosa of Siemens
• Epithelial keratin-2e
• K2e
• keratin 2
• keratin 2A (epidermal ichthyosis bullosa of Siemens)
• keratin, type II cytoskeletal 2 epidermal
• Keratin-2 epidermis
• keratin-2e
• KRT2AMGC116967
• KRT2EMGC116968
• KRTE
• Type-II keratin Kb2

Background

Cytokeratins, a group of at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 4, 5, 6 and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin peptide 4 (59 kDa) is the secondary type II keratin expressed in non cornified stratified squamous epithelia. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia, while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. Cytokeratins 10, 13 and 18 are members of the type I acidic subfamily. Cytokeratin peptide 10 (56 kDa) is the secondary type I keratin expressed in cornified epithelia. Cytokeratin 13 (54 kDa) is the secondary type I keratin expressed in non-cornified stratified squamous epithelia. Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells. Cytokeratin is a heterotetramer of two type I and two type II keratins. Keratin 1 is generally associated with keratin 10. Defects in Cytokeratin are a cause of epidermolytic hyperkeratosis, also known as bullous congenital ichthyosiform erythroderma (BIE), which is a hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. Defects in Cytokeratin are also the cause of Curth Macklin type ichthyosis hystrix, nonepidermolytic palmoplantar keratoderma, annular epidermolytic ichthyosis and keratosis palmoplantaris striata III (PPKS3). PPKS3 is an autosomal dominant disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of nonpalmoplantar skin, and both hair and nails are normal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Cytokeratin 2 Antibody (NBP1-31423)(1)

Publication using NBP1-31423

• Zlatar, L;Timm, T;Lochnit, G;Bilyy, R;Bäuerle, T;Munoz-Becerra, M;Schett, G;Knopf, J;Heichel, J;Ali, MJ;Schapher, M;Paulsen, F;Herrmann, M; Neutrophil Extracellular Traps Drive Dacryolithiasis Cells 2023-07-14 [PMID: 37508521] (Immunohistochemistry)

Bioinformatics

• Gene Symbol: KRT2