Cytokeratin 19 Antibody (4E8) - Azide and BSA Free

Western Blot: Cytokeratin 19 Antibody (4E8) - Azide and BSA Free [NBP2-80689] - Western blot analysis using Cytokeratin 19 mouse mAb against T47D (1), MCF-7 (2), HepG2 (3) and SW620 (4) cell lysate. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (4E8) - Azide and BSA Free [NBP2-80689] - Immunofluorescence analysis of HepG2 cells using Cytokeratin 19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.

Immunohistochemistry: Cytokeratin 19 Antibody (4E8) - Azide and BSA Free [NBP2-80689] - Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using Cytokeratin 19 mouse mAb with DAB staining. Image from the standard format of this antibody.

Western Blot: Cytokeratin 19 Antibody (4E8) - Azide and BSA Free [NBP2-80689] - Western blot analysis using Cytokeratin 19 mAb against human Cytokeratin 19 (AA: 115-269) recombinant protein. (Expected MW is 43.1 kDa) Image from the standard format of this antibody.

Immunohistochemistry: Cytokeratin 19 Antibody (4E8) - Azide and BSA Free [NBP2-80689] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using Cytokeratin 19 mouse mAb with DAB staining. Image from the standard format of this antibody.

Immunohistochemistry: Cytokeratin 19 Antibody (4E8) - Azide and BSA Free [NBP2-80689] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Cytokeratin 19 mouse mAb with DAB staining. Image from the standard format of this antibody.

ELISA: Cytokeratin 19 Antibody (4E8) - Azide and BSA Free [NBP2-80689] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng). Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Cytokeratin 19 Antibody (4E8) - Azide and BSA Free Summary

• Immunogen: Purified recombinant fragment of human Cytokeratin 19 expressed in E. coli. [UniProt# P08727]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT19
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:25-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:500-1:2000
• Application Notes: This Cytokeratin 19 (4E8) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA.
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Cytokeratin 19 Antibody (4E8) - Azide and BSA Free

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT19