Cytokeratin 15 Antibody (6E7)

Western Blot: Cytokeratin 15 Antibody (6E7) [NBP1-51587] - Western blot analysis using KRT15 mouse mAb against A431 cell lysate.

Immunocytochemistry/Immunofluorescence: Cytokeratin 15 Antibody (6E7) [NBP1-51587] - Immunofluorescence analysis of HepG2 (left) and PACN-1 (right) cells using KRT15 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (6E7) [NBP1-51587] - Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using KRT15 mouse mAb.

Flow Cytometry: Cytokeratin 15 Antibody (6E7) [NBP1-51587] - Flow cytometric analysis of PACN-1 cells using KRT15 mouse mAb (green) and negative control (purple).

Product Discontinued

Cytokeratin 15 Antibody (6E7) Summary

• Immunogen: Purified recombinant fragment of KRT15 expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT15
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Cytokeratin 15 Antibody (6E7)

• CK15
• CK-15
• cytokeratin 15
• Cytokeratin-15
• K15keratin-15, basic
• K1CO
• keratin 15
• keratin, type I cytoskeletal 15
• Keratin-15
• keratin-15, beta
• KRTB
• type I cytoskeletal 15

Background

A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues (1). Cytokeratin 15 (CK15) is a type I keratin without a defined type II partner whose expression in epidermal diseases has not been investigated. A monoclonal antibody raised against the last 17 amino acids of the CK15 polypeptide show that CK-15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Although CK-15 in normal hair follicles was virtually absent from hair bulbs, it was expressed by a subset of keratinocytes in the outer root sheath (2). In human conjunctival epithelium, strong expression of CK-15 was observed in basal cells, whereas CK19 was expressed in both basal and suprabasal layers. Although the expression of K15 and K19 differ in humans and mice, specific staining patterns can be used to characterize the epithelial phenotype in normal and diseased ocular surface (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT15