Recombinant Human Cytokeratin 14 His Protein

SDS-PAGE: Recombinant Human Cytokeratin 14 His Protein [NBP1-72467] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

SDS-PAGE: Recombinant Human Cytokeratin 14 His Protein [NBP1-72467] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Cytokeratin 14 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-472 of Human Cytokeratin 14
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIK DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN LEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: KRT14
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 53.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human Cytokeratin 14 His Protein

• CK14
• CK-14
• Cytokeratin 14
• cytokeratin-14
• EBS3
• EBS4
• K14
• keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
• keratin 14
• keratin, type I cytoskeletal 14
• keratin-14
• KRT14
• NFJ

Background

KRT14, also known as keratin 14, is a member of the cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3 sigma to mediate protein synthesis. Recombinant human KRT14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: KRT14