Cytokeratin 14 Antibody (SP53)

Images

 
Western Blot: Cytokeratin 14 Antibody (SP53) [NBP1-49816] A431
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SP53) [NBP1-49816] - Human Prostate

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
SP53
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cytokeratin 14 Antibody (SP53) Summary

Immunogen
Synthetic peptide corresponding to C-terminus of human keratin 14 protein
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
KRT14
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This product is useful for Immunohistochemistry Paraffin. Antigen retrieval can be performed using a Citrate process.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
7.0 ml prediluted antibody with 10mM PBS (pH 7.4) and 1.0% BSA
Preservative
Sodium Azide
Purity
Protein A or G purified

Notes

For a positive control use Colon adenocarcinoma.

Alternate Names for Cytokeratin 14 Antibody (SP53)

  • CK14
  • CK-14
  • Cytokeratin 14
  • cytokeratin-14
  • EBS3
  • EBS4
  • K14
  • keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
  • keratin 14
  • keratin, type I cytoskeletal 14
  • keratin-14
  • KRT14
  • NFJ

Background

Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer. This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT14
Entrez