Cytokeratin 14 Antibody (SP53)

Product Discontinued

Cytokeratin 14 Antibody (SP53) Summary

• Immunogen: Synthetic peptide corresponding to C-terminus of human keratin 14 protein
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT14
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This product is useful for Immunohistochemistry Paraffin. Antigen retrieval can be performed using a Citrate process.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 7.0 ml prediluted antibody with 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: Sodium Azide
• Purity: Protein A or G purified

Notes

For a positive control use Colon adenocarcinoma.

Alternate Names for Cytokeratin 14 Antibody (SP53)

• CK14
• CK-14
• Cytokeratin 14
• cytokeratin-14
• EBS3
• EBS4
• K14
• keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
• keratin 14
• keratin, type I cytoskeletal 14
• keratin-14
• KRT14
• NFJ

Background

Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer. This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT14