Cytokeratin 14 Antibody (DE-SPK14)

Product Discontinued

Cytokeratin 14 Antibody (DE-SPK14) Summary

• Immunogen: The antibody reacts with the Keratin 14 antigen.
• Specificity: DE-SPK14 reacts specifically in immunoblotting with human keratin 14 (50 kD). In tissue sections this mAb provides a positive reaction on basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingival, myoepithelial cells and squamous cell carcinomas.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT14
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: Suitable for frozen sections / cell preparations. Also suitable for paraffin-embedded tissue when using the MICROWAVE method.Working dilution: 1:20, based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but optimal dilution should be tested by serial dilution. Dilute immediately before use with PBS or Tris. Positive control: Glandular

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: No Preservative
• Purity: Tissue culture supernatant

Alternate Names for Cytokeratin 14 Antibody (DE-SPK14)

• CK14
• CK-14
• Cytokeratin 14
• cytokeratin-14
• EBS3
• EBS4
• K14
• keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
• keratin 14
• keratin, type I cytoskeletal 14
• keratin-14
• KRT14
• NFJ

Background

Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer. This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT14