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Cytokeratin 10 Antibody (KRT10/844 + KRT10/1275)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
KRT10/844 + KRT10/1275
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54327AF350
Alexa Fluor 405
NBP2-54327AF405
Alexa Fluor 488
NBP2-54327AF488
Alexa Fluor 532
NBP2-54327AF532
Alexa Fluor 594
NBP2-54327AF594
Alexa Fluor 647
NBP2-54327AF647
Alexa Fluor 700
NBP2-54327AF700
Alexa Fluor 750
NBP2-54327AF750
Biotin
NBP2-54327B
DyLight 350
NBP2-54327UV
DyLight 405
NBP2-54327V
DyLight 488
NBP2-54327G
DyLight 550
NBP2-54327R
DyLight 594
NBP2-54327DL594
DyLight 650
NBP2-54327C
DyLight 680
NBP2-54327FR
DyLight 755
NBP2-54327IR
FITC
NBP2-54327F
HRP
NBP2-54327H
Janelia Fluor 549
NBP2-54327JF549
Janelia Fluor 646
NBP2-54327JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-54327-100ug
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Cytokeratin 10 Antibody (KRT10/844 + KRT10/1275) Summary

Immunogen
Recombinant human Cytokeratin 10 protein (Uniprot: P13645)
Localization
Cytoplasmic
Marker
Suprabasal Epithelial Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT10
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.1-0.2 ug/ml
  • Western Blot 1:2000
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54327)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 10 Antibody (KRT10/844 + KRT10/1275)

  • BCIE
  • BIE
  • CK10
  • CK-10
  • cytokeratin 10
  • Cytokeratin-10
  • EHK
  • K10keratosis palmaris et plantaris
  • Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris)
  • keratin 10
  • keratin, type I cytoskeletal 10
  • Keratin-10
  • KPP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Dual RNAscope ISH-IHC: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - FFPE tissue sections of human metastatic tonsil were probed for Pan Cytokeratin mRNA (ACD RNAScope probe, catalog # 310221; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for IHC using mouse monoclonal antibody (Novus catalog # NBP2-29429) at 0.3 ug/mL for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Novus Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).
CyTOF-reported: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Human fibroblasts. Blue: fibroblasts. Red: fibroblast + cytokeratin transfection. CyTOF image submitted by a verified customer review.
Flow Cytometry: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Human Pan-Cytokeratins on HeLa Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Pan-Cytokeratin Monoclonal Antibody (AE-1/AE-3)
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Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
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Involucrin
Antibody (SY5) - BSA
NB100-2727
Immunohistochemistry-Paraffin: Involucrin Antibody (SY5) [NB100-2727] - Staining of human Tonsil with Involucrin Monoclonal Antibody (SY5).
Western Blot: Involucrin Antibody (SY5) [NB100-2727] - Involucrin from primary human epidermal keratinocyte lysates grown in low calcium (0.07 mM) for 5 days or differentiated with high calcium (1.5 mM) for 1-5 days. Block in 5% nonfat dry milk in TBST incubated at RT for 1 hr, antibody 1:500 in TBST, overnight at 4C. Image from verified customer review.
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Cytokeratin 19
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NB100-687
Flow Cytometry: Cytokeratin 19 Antibody [NB100-687] - An intracellular stain was performed on MCF7 cells with Cytokeratin 19 Antibody NB100-687AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.
Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Inhibition of MST improves liver regeneration in aged mice. Representative photomicrograph of an aged liver treated with siMST and stained with H&E and by IHC for CK19 and Ki67. Arrows indicate ductal regions with no signs of reaction or oval cell expansion. Image collected and cropped by CiteAb from the following publication (http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.
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Cytokeratin 16
Antibody (OTI2H8)
NBP2-45538
Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu, Rt
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Cytokeratin 5
Antibody (EP1601Y)
NB110-56916
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Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody [NBP2-38166] - Staining of human prostate shows positivity in the basal layer.
Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody [NBP2-38166] - Staining of human skin shows positivity in epidermal cells.
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Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
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Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human cervix shows moderate to strong positivity in mucus in glandular cells.
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Choline Kinase
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Choline Kinase beta [Unconjugated]
Species: Hu
Applications: EnzAct
Cytokeratin 7
Antibody (OV-TL12/30) ...
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Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Lane view shows a specific band for Cytokeratin 7 at 5 ug/mL in HeLa cell lysate.Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 61 kDa. Image from an internal validation.
Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/mL dilution of HeLa lysates(s) respectively.
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Kv6.1 Antibody
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Immunohistochemistry-Paraffin: Kv6.1 Antibody [NBP1-81572] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunocytochemistry/Immunofluorescence: Kv6.1 Antibody [NBP1-81572] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
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Vimentin Antibody
NB300-223
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Analysis of HeLa cell culture. Antibody at 1:10,000 in green, and costained with mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - 10 um Cryosections from PFA fixed murine brain acute slices stained with Vimentin antibody (1:600) and Alexa Fluor 488 anti chicken IgG. Antibody showed specific staining, also of blood vessels. IHC-Fr image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Human A7 cells. Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4C O/N followed with Alexa Fluor 488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Imaged with Leica TCS SP5 confocal microscope. ICC/IF image submitted by a verified customer review.
(6 Reviews)
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Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
HOXB2 Antibody
(PCRP-HOXB2-1F2)
NBP3-13937
Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Flow cytometric analysis of PFA-fixed HeLa cells. HOXB2 antibody (PCRP-HOXB2-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunocytochemistry/Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HOXB2 Antibody (PCRP-HOXB2-1F2). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC-P, IP, PA, WB

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Bioinformatics

Gene Symbol KRT10
Uniprot