Cytokeratin 10 Antibody (KRT10/844 + KRT10/1275)

Images

 
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (KRT10/844 + KRT10/1275) [NBP2-44709] - Human Tonsil stained with Cytokeratin 10 Monoclonal Antibody (KRT10/844 + KRT10/1275).
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (KRT10/844 + KRT10/1275) [NBP2-44709] - Formalin-fixed, paraffin-embedded human skin stained with cytokeratin 10 Monoclonal Antibody (KRT10/+KRT10/1275)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-P, ICC/IF
Clone
KRT10/844 + KRT10/1275
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 10 Antibody (KRT10/844 + KRT10/1275) Summary

Immunogen
Recombinant human KRT10 protein
Localization
Cytoplasmic
Marker
Suprabasal Epithelial Marker
Specificity
This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT10
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 17q21.2
Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
Theoretical MW
56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytokeratin 10 Antibody (KRT10/844 + KRT10/1275)

  • BCIE
  • BIE
  • CK10
  • CK-10
  • cytokeratin 10
  • Cytokeratin-10
  • EHK
  • K10keratosis palmaris et plantaris
  • Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris)
  • keratin 10
  • keratin, type I cytoskeletal 10
  • Keratin-10
  • KPP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-54327AF350
Alexa Fluor 405 NBP2-54327AF405
Alexa Fluor 488 NBP2-54327AF488
Alexa Fluor 532 NBP2-54327AF532
Alexa Fluor 594 NBP2-54327AF594
Alexa Fluor 647 NBP2-54327AF647
Alexa Fluor 700 NBP2-54327AF700
Alexa Fluor 750 NBP2-54327AF750
Allophycocyanin NBP2-54327APC
Biotin NBP2-54327B
DyLight 350 NBP2-54327UV
DyLight 405 NBP2-54327V
DyLight 488 NBP2-54327G
DyLight 550 NBP2-54327R
DyLight 594 NBP2-54327DL594
DyLight 650 NBP2-54327C
DyLight 680 NBP2-54327FR
DyLight 755 NBP2-54327IR
FITC NBP2-54327F
HRP NBP2-54327H
Janelia Fluor 549 NBP2-54327JF549
Janelia Fluor 646 NBP2-54327JF646
PE NBP2-54327PE
PerCP NBP2-54327PCP

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Bioinformatics

Gene Symbol KRT10
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