Cytokeratin 1 Antibody (3C10F7)

Western Blot: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - Analysis using CK1 mouse mAb against truncated CK1 recombinant protein.

Immunocytochemistry/Immunofluorescence: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Cytokeratin 1 (3C10F7) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - Analysis of human liver tissue (A), colon carcinoma (B), lung carcinoma (C) and esophagus tissue (D), showing membrane localization using CK1 mouse mAb with DAB staining.

• Reactivity: Hu, Po, Bv, Ca, Eq, Fe, Gt
• Applications: WB, ELISA, ICC/IF, IHC, IHC
• Clone: 3C10F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cytokeratin 1 Antibody (3C10F7) Summary

• Description: Novus Biologicals Mouse Cytokeratin 1 Antibody (3C10F7) (NB110-89450) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Cytokeratin 1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of Cytokeratin 1 expressed in E. Coli.
• Specificity: CK1 (PMC01)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: Use in Immunohistochemistry free floating reported in scientific literature (PMID: 26190143).

Reactivity Notes

Porcine, Bovine, Goat, Equine, Canine, and Feline reactivities reported in scientific literature (PMID: 26190143).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Cytokeratin 1 Antibody (3C10F7)

• CK1
• CK-1
• cytokeratin 1
• Cytokeratin-1
• cytokeratin-1,67 kDa cytokeratin
• EHK
• EHK1
• epidermolytic hyperkeratosis 1
• EPPK
• Hair alpha protein
• K1
• keratin 1
• keratin, type II cytoskeletal 1
• Keratin-1
• KRT1A
• KRTA
• NEPPK
• Type-II keratin Kb1

Background

CK1 (also designated Cytokeratin 1 or KRT1 ),with 644-amino acid protein (about 70kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells.CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 1 Antibody (NB110-89450)(2)

Publications using NB110-89450

• Fuchs C, Stalnaker KJ, Dalgard CL et al. Plantar Skin Exhibits Altered Physiology, Constitutive Activation of Wound-Associated Phenotypes, and Inherently Delayed Healing. The Journal of investigative dermatology 2024-06-22 [PMID: 38237729]
• Nautscher N, Bauer A, Steffl M, Amselgruber WM et al. Comparative morphological evaluation of domestic animal cornea Vet Ophthalmol. 2015-07-20 [PMID: 26190143] (IHC-P, Equine, Feline, Goat, Bovine, Canine, Porcine)
  
  Details:
  Cytokeratin 1 antibody used for Immunohistochemistry on paraffin embedded corneal sections from pig, cow, goat, horse, dog and cat.

Bioinformatics

• Gene Symbol: KRT1
• Entrez:
  • 3848(Human)