Cytochrome P450 2E1 Antibody (OTI5B9)

Western Blot: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2E1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2E1.

Immunocytochemistry/Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) [NBP2-02155] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2E1.

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Staining of paraffin-embedded Ovary tissue using anti-Cytochrome P450 2E1 mouse monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Immunofluorescent staining of HepG2 cells using anti-Cytochrome P450 2E1mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Staining of paraffin-embedded endometrium tissue using anti-Cytochrome P450 2E1 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Func, ICC/IF, IHC
• Clone: OTI5B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.53 mg/ml

Cytochrome P450 2E1 Antibody (OTI5B9) Summary

• Description: Novus Biologicals Mouse Cytochrome P450 2E1 Antibody (OTI5B9) (NBP2-02155) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CYP2E1 (NP_000764) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYP2E1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunodiffusion
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Theoretical MW: 56.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.53 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytochrome P450 2E1 Antibody (OTI5B9)

• 4-nitrophenol 2-hydroxylase
• CPE1
• CYP2Ecytochrome P450 2E1
• CYPIIE1
• cytochrome P450, family 2, subfamily E, polypeptide 1
• cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1
• Cytochrome P450-J
• EC 1.14.13.-
• EC 1.14.13.n7
• EC 1.14.14.1
• flavoprotein-linked monooxygenase
• microsomal monooxygenase
• P450C2E
• P450-J
• xenobiotic monooxygenase

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP2E1
• Entrez:
  • 1571(Human)
• Uniprot:
  • P05181(Human)