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Cytochrome P450 2D6 Antibody (JM44-23)

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Western Blot: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1,000 dilution.
Western Blot: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Staining Cytochrome P450 2D6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunohistochemistry-Paraffin: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Staining Cytochrome P450 2D6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunohistochemistry-Paraffin: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.
Simple Western: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Lane 25 is a Human Liver Tissue Lysate Sample (African American) blotted with NBP2-67020 and shows a clear CYP2D6 red band around a molecular weight ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Clone
JM44-23
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Cytochrome P450 2D6 Antibody (JM44-23) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant Protein (SwissProt: P10635 Human)
Localization
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CYP2D6
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Simple Western
  • Western Blot 1:500-1:2000
Reviewed Applications
Read 1 Review rated 5
using
NBP2-67020 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Cytochrome P450 2D6 Antibody (JM44-23)

  • CPD6P450DB1
  • CYP2D
  • CYP2D6
  • CYP2D7AP
  • CYP2D7BP
  • CYP2D7P2
  • CYP2D8P2
  • CYP2DL1
  • CYPIID6
  • Cytochrome P450 2D6
  • cytochrome P450, family 2, subfamily D, polypeptide 6
  • cytochrome P450, family 2, subfamily D, polypeptide 7 pseudogene 2
  • cytochrome P450, family 2, subfamily D, polypeptide 8 pseudogene 2
  • cytochrome P450, subfamily II (debrisoquine, sparteine, etc., -metabolising)
  • cytochrome P450, subfamily IID (debrisoquine, sparteine, etc.
  • cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolising)
  • cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolizing)
  • Cytochrome P450-DB1
  • Debrisoquine 4-hydroxylase
  • EC 1.14.14.1
  • flavoprotein-linked monooxygenase
  • -metabolizing)-like 1
  • MGC120389
  • MGC120390
  • microsomal monooxygenase
  • P450C2D
  • P450-DB1
  • polypeptide 6
  • polypeptide 7 pseudogene 2
  • polypeptide 8 pseudogene 2
  • xenobiotic monooxygenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ELISA: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)
Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
(1 Publication)
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Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
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Western Blot: Cytochrome P450 2C19 Antibody [NBP3-10546] - Western blot analysis using NBP3-10546 on Human Placenta as a positive control. Antibody Titration: 0.2-1 ug/ml
Species: Hu
Applications: WB
Cytochrome P450
2C9 Antibody (OTI1D7)
NBP2-01397
Flow Cytometry: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cytochrome P450 2C9 antibody, and then analyzed by flow cytometry.
Western Blot: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2C9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2C9.
Immunohistochemistry-Paraffin: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Cytochrome P450 2C9 mouse monoclonal antibody.
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Cytochrome P450
1A2 Antibody (OTI8F1)
NBP2-02563
Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Human colon tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Cytochrome P450
2E1 Antibody
NBP1-85367
Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85367] - Western blot analysis in mouse liver tissue and rat liver tissue.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Staining of human endometrium, liver, liver cancer and tonsil using Anti-CYP2E1 antibody NBP1-85367 (A) shows similar protein distribution across tissues to independent antibody NBP1-85366 (B).
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Analysis in human liver and endometrium tissues. Corresponding CYP2E1 RNA-seq data are presented for the same tissues.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt, V-Vi
Applications: IHC, IHC-P, WB
Cytochrome P450
2A6 Antibody (OTI1D2)
NBP2-01437
Western Blot: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2A6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2A6.
Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Human bladder tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.
Western Blot: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2A6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Species: Hu, Pm, Mu, Pm, Rt
Applications: Flow, IHC, IHC-P, WB
Cytochrome P450
1A1/1A2 Antibody (MC1
NB100-74398
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescentred phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
(1 Review)
(2 Publications)
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PRB1 Antibody
NBP3-10064
Western Blot: PRB1 Antibody [NBP3-10064] - Western blot analysis of PRB1 in Human Hela Whole Cell lysates. Antibody dilution at 1ug/ml
Species: Hu
Applications: WB
Cytochrome P450
2B6 Antibody (OTI3D5)
NBP2-01800
Flow Cytometry: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Analysis of Jurkat cells, using anti-Cytochrome P450 2B6 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cytochrome P450 2B6 antibody, and then analyzed by flow cytometry.
Immunocytochemistry/Immunofluorescence: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2B6.
(1 Publication)
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
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2C8 Antibody
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Western Blot: Cytochrome P450 2C8 Antibody [NBP1-88054] - Analysis using Anti-CYP2C8 antibody NBP1-88054 (A) shows similar pattern to independent antibody NBP1-88055 (B).
Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Analysis in human liver and skin tissues. Corresponding CYP2C8 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.
Species: Hu
Applications: IHC, IHC-P, WB
GST Epitope Tag
Antibody - BSA Free
NB600-326
Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).
Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hUbiquitn-GST (left) & hISG15-GST (right) lysate(s).
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Cytochrome P450
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Immunohistochemistry: Cytochrome P450 3A5 Antibody (F18P3B6) [NBP2-50209] - Staining of CYP3A5 in an oesophagus cancer cross section.
Western Blot: Cytochrome P450 3A5 Antibody (F18P3B6) [NBP2-50209] - Western Blot of recombinant CYP450s
(1 Review)
Species: Hu
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Cytochrome P450
2C18 Antibody
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Western Blot: Cytochrome P450 2C18 Antibody [NBP2-92808] - Analysis of extracts of various cell lines, using Cytochrome P450 2C18 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Species: Hu, Mu, Rt
Applications: WB
MYH Antibody (4D10)
H00004595-M01
Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.Lane 1: MUTYH transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
Knockdown Validated: MYH Antibody (4D10) [H00004595-M01] - Simultaneous suppression of MTH1 and MYH was efficiently achieved using a two-vector system. To investigate the knockdown efficiency at protein level, western blot analysis was performed after 96 hrs of Dox treatment of cells with shRNA set1 (d). Data were double normalized with GAPDH and NT-shRNA control samples and expressed as percentage. The graphs represent mean +/-s.e.m. from three independent experiments. P-values were calculated using one-way analysis of variance (ANOVA). *P<0.05, **P<0.01, ***P<0.001, NS, not significant. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/oncsis201672) licensed under a CC-BY licence.
Immunoprecipitation: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH MaxPab rabbit polyclonal antibody.
(10 Publications)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, KD, WB
MDR1/ABCB1
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Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.
Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Mouse (B6) liver tissue. Fixed in ice-cold methanol for 10 minutes. Blocking in 10% serum and 1% BSA. Primary antibody at 5 ug/mL, overnight incubation. Secondary antibody at 2 ug/mL for 1.5 hour (Invitrogen A-11012). Mounting with Vector Vectashield with DAPI. Image from a verified customer review.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded mouse kidney tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NAT2 Antibody
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Immunohistochemistry-Paraffin: NAT2 Antibody [NBP1-33751] - A549 xenograft, using NAT2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: NAT2 Antibody [NBP1-33751] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 12% SDS PAGE; antibody diluted at 1:1000.
Species: Hu
Applications: IHC, IHC-P, WB
SLC38A1 Antibody
(S104-32)
NBP2-59311
Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32 (NBP2-59311). Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody (NBP2-59311) at 1:1000.
Immunocytochemistry/Immunofluorescence: SLC38A1 Antibody (S104-32) [NBP2-59311] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone S104-32 (NBP2-59311). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody (NBP2-59311) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SNAT1 Antibody (D) Composite.
Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32. Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody at 1:1000.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NARG1L Antibody
NBP1-92167
Western Blot: NARG1L Antibody [NBP1-92167] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: NARG1L Antibody [NBP1-92167] - Staining of human appendix shows moderate cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: NARG1L Antibody [NBP1-92167] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
GSTT1 Antibody
NBP1-32733
Immunohistochemistry-Paraffin: GSTT1 Antibody [NBP1-32733] - Human hepatoma. GSTT1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: GSTT1 Antibody [NBP1-32733] - Sample (30 ug of whole cell lysate)A: Hep G2 10% SDS PAGE, antibody diluted at 1:1000.
Immunoprecipitation: GSTT1 Antibody [NBP1-32733] - Immunoprecipitation of GSTT1 protein from HepG2 whole cell extracts using 5 ug of GSTT1 antibody. Western blot analysis was performed using GSTT1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Cytochrome P450
2D6 Antibody (JM44-23
NBP2-67020
Cytochrome P450 2D6 Antibody (JM44-23)
Species: Hu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P

Publications for Cytochrome P450 2D6 Antibody (NBP2-67020) (0)

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Review for Cytochrome P450 2D6 Antibody (NBP2-67020) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-67020:
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 On Jess from Protein Simple Human 11/07/2019
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Summary

ApplicationOther
Sample Testedhuman liver cell,Human tissue
SpeciesHuman
LotHK0703

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Bioinformatics

Gene Symbol CYP2D6