![Western Blot: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Cytochrome-P450-2D6-Antibody-JM44-23-Western-Blot-NBP2-67020-img0004.jpg "Western Blot: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1,000 dilution.")
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within Human Cytochrome P450 2D6 aa 301-497 / 497. (SwissProt: P10635 Human) |
| Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CYP2D6 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP2-67020 | Applications | Species |
|---|---|---|
| Collins JM, Lester H, Shabnaz S, Wang D A frequent CYP2D6 variant promotes skipping of exon 3 and reduces CYP2D6 protein expression in human liver samples Frontiers in pharmacology 2023-07-27 [PMID: 37576811] (Simple Western, Human) | Simple Western | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Dahao Ling |
On Jess from Protein Simple | Human | 11/07/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytochrome P450 2D6 Antibody (NBP2-67020)Find related products by research area.
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| Dahao Ling 11/07/2019 |
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| Application: | On Jess from Protein Simple | |
| Species: | Human |
| Gene Symbol | CYP2D6 |
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Staining Cytochrome P450 2D6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-2D6-Antibody-JM44-23-Immunocytochemistry-Immunofluorescence-NBP2-67020-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Staining Cytochrome P450 2D6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-2D6-Antibody-JM44-23-Immunohistochemistry-Paraffin-NBP2-67020-img0003.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Staining Cytochrome P450 2D6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-2D6-Antibody-JM44-23-Immunocytochemistry-Immunofluorescence-NBP2-67020-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Staining Cytochrome P450 2D6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-2D6-Antibody-JM44-23-Immunohistochemistry-Paraffin-NBP2-67020-img0002.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.")
![Simple Western: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Lane 25 is a Human Liver Tissue Lysate Sample (African American) blotted with NBP2-67020 and shows a clear CYP2D6 red band around a molecular weight of 51KDa. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytochrome-P450-2D6-Antibody-JM44-23-Simple-Western-NBP2-67020-img0006.jpg "Simple Western: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Lane 25 is a Human Liver Tissue Lysate Sample (African American) blotted with NBP2-67020 and shows a clear CYP2D6 red band around a molecular weight of 51KDa. Simple Western image submitted by a verified customer review.")
![Simple Western: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Testing mRNA and protein stabilities of full-length CYP2D6 and its splice isoforms. (A) Time course of the relative expression levels of full-length CYP2D6, CYP2D6 E3, and CYP2D6 E6 in HepG2 cells after actinomycin D treatment. (B) Western blot image of CYP2D6 protein isoforms in HEK293 T cells transfected with plasmid DNAs expressing full-length (HEK2D6F), CYP2D6 E3 (HEK2D6S), a mix of the two (mix-2D6F/2D6S), or a mix of the two and treated with cycloheximide (Cyclo-mix-2D6F/2D6S). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37576811 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-67020_rabbit-monoclonal-cytochrome-p450-2d6-antibody-jm44-23-308202410445510.jpg "Simple Western: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Testing mRNA and protein stabilities of full-length CYP2D6 and its splice isoforms. (A) Time course of the relative expression levels of full-length CYP2D6, CYP2D6 E3, and CYP2D6 E6 in HepG2 cells after actinomycin D treatment. (B) Western blot image of CYP2D6 protein isoforms in HEK293 T cells transfected with plasmid DNAs expressing full-length (HEK2D6F), CYP2D6 E3 (HEK2D6S), a mix of the two (mix-2D6F/2D6S), or a mix of the two and treated with cycloheximide (Cyclo-mix-2D6F/2D6S). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37576811 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
