Cytochrome P450 2A6 Antibody

Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody [NBP2-33485] - Analysis in human liver and skeletal muscle tissues using NBP2-33485 antibody. Corresponding Cytochrome P450 2A6 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody [NBP2-33485] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody [NBP2-33485] - Staining of human liver shows strong ctyoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody [NBP2-33485] - Staining of human kidney shows ver weak cytoplasmic positivity in cells in tubules as expected.

Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody [NBP2-33485] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Simple Western: Cytochrome P450 2A6 Antibody [NBP2-33485] - Lane view shows a specific band for Cytochrome P450 2A6 in Liver lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 54 kDa. Image from an internal validation.

Product Discontinued

Cytochrome P450 2A6 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: QEEAGFLIDALRGTGGANIDPTFFLSR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP2A6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:25
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: tested in liver lysates; separated by size; antibody dilution of 1:25; Apparent MW in kDa on Simple Western was ~55 kDa

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Cytochrome P450 2A6 Antibody

• Coumarin 7-hydroxylase
• CPA6
• CYP2A3P450C2A
• CYP2AEC 1.14.14.1
• CYPIIA6
• cytochrome P450 2A6
• Cytochrome P450 IIA3
• Cytochrome P450(I)
• cytochrome P450, family 2, subfamily A, polypeptide 6
• cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 6
• flavoprotein-linked monooxygenase
• P450PB
• xenobiotic monooxygenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP2A6
• Uniprot:
  • P11509(Human)