Cytochrome c Antibody (7H8.2C12 + CYCS/1010)

Images

 
Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12 + CYCS/1010) [NBP2-44447] - Human Pancreas stained with Cytochrome C Monoclonal Antibody (7H8.2C12 + CYCS/1010).
Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12 + CYCS/1010) [NBP2-44447] - Human Heart stained with Cytochrome C Monoclonal Antibody (7H8.2C12 + CYCS/1010).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, IHC, IHC-P, ICC/IF
Clone
7H8.2C12 + CYCS/1010
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytochrome c Antibody (7H8.2C12 + CYCS/1010) Summary

Immunogen
Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c (7H8.2C12); Recombinant full-length human CYCS protein (CYCS/1010)
Localization
Cytoplasmic
Marker
Mitochondrial Marker
Specificity
Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
CYCS
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 7p15.3
Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytochrome c Antibody (7H8.2C12 + CYCS/1010)

  • CYCHCS
  • CYCS
  • Cytochrome c
  • cytochrome c, somatic
  • THC4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytochrome c Antibody (NBP2-44447) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Cytochrome c Antibody (NBP2-44447) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47694AF350
Alexa Fluor 405 NBP2-47694AF405
Alexa Fluor 488 NBP2-47694AF488
Alexa Fluor 532 NBP2-47694AF532
Alexa Fluor 594 NBP2-47694AF594
Alexa Fluor 647 NBP2-47694AF647
Alexa Fluor 700 NBP2-47694AF700
Alexa Fluor 750 NBP2-47694AF750
Allophycocyanin NBP2-47694APC
Biotin NBP2-47694B
DyLight 350 NBP2-47694UV
DyLight 405 NBP2-47694V
DyLight 488 NBP2-47694G
DyLight 550 NBP2-47694R
DyLight 594 NBP2-47694DL594
DyLight 650 NBP2-47694C
DyLight 680 NBP2-47694FR
DyLight 755 NBP2-47694IR
FITC NBP2-47694F
HRP NBP2-47694H
Janelia Fluor 549 NBP2-47694JF549
Janelia Fluor 646 NBP2-47694JF646
PE NBP2-47694PE
PE NBP2-44447PE
PerCP NBP2-47694PCP

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Blogs on Cytochrome c.

Pathway Highlight: Three key factors that contribute to cellular heterogeneity in apoptosis
Have you ever wondered why cells in the same population respond differently to an apoptotic stimulus? Apoptosis, a form of programmed cell death, is vital for the removal of unwanted or damaged cells. As with most cellular processes, too much or to...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

Cytochrome C in Apoptosis, Immune Response and Cancer
Cytochrome C is an electron carrier protein that localizes in mitochondrion intermembrane space and has been identified as one of the key signaling molecules of apoptosis or programmed cell death. Suppression of the anti-apoptotic members or activatio...  Read full blog post.

Mitofilin and the Mitochondrial Inner Membrane Organizing System (MINOS)
Mitofilin was originally described as a heart muscle protein due to its high expression in the heart. Recently, analysis of the human heart mitochondrial proteome demonstrated that Mitofilin is one of the most abundant mitochondrial proteins (1). Rese...  Read full blog post.

Bax Research Gives New Insight into Oxidative Apoptosis
Bax is a member of the Bcl-2 family; an extensive range of proteins which play key roles in apoptosis, or programmed cell death, by regulating outer mitochondrial membrane permeability. We at Novus Biologicals are one of the leading antibody suppliers...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

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Bioinformatics

Gene Symbol CYCS
Uniprot