Cystatin E/M/CST6 Antibody

Immunohistochemistry: Cystatin E/M/CST6 Antibody [28730002] - Most of the normal tissues displayed weak to moderate cytoplasmic positivity. Strong staining was observed in a fraction of cells in renal tubules and in stratum granulosum of squamous epithelia. Liver and urothelial cells were mainly negative. Malignant tissues generally exhibited similar staining properties as normal cells. Occasional cases of renal cancers showed strong nuclear immunoreactivity. Most malignant lymphomas, prostate and hepatocellular cancers were negative. Image and statement courtesy of the Human Protein Atlas (HPA).

ELISA: Cystatin E/M/CST6 Antibody [28730002]

Product Discontinued

Cystatin E/M/CST6 Antibody Summary

• Immunogen: This antibody is specific for the entire range of the target protein.
• Specificity: This product is specific for Human CST6.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CST6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This antibody is useful in ELISA and Immunohistochemistry-Paraffin.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for Cystatin E/M/CST6 Antibody

• CST6
• cystatin 6
• Cystatin E/M
• cystatin M
• cystatin M/E
• Cystatin-6
• cystatin-E
• cystatin-M
• cysteine proteinase inhibitor

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cystatin E/M/CST6 Antibody (28730002)(2)

Publications using 28730002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: CST6
• Uniprot:
  • Q15828()