Cystatin E/M/CST6 Antibody (211515) [Unconjugated]

Western blot shows lysates of human skin tissue and human colon cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cystatin E/M Monoclonal Antibody (Catalog # MAB1286) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cystatin E/M at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cystatin E/M was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human Cystatin E/M Monoclonal Antibody (Catalog # MAB1286) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in hair follicules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP, B/N
• Clone: 211515
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cystatin E/M/CST6 Antibody (211515) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cystatin E/M
  Arg29-Met149
  Accession # Q15828
• Specificity: Detects human Cystatin E/M in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cystatins A, B, C, S, SA, SN, rhFetuin A, B, rhHRGP, or rhKininogen is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CST6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 7 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin E/M/CST6 Antibody (211515) [Unconjugated]

• CST6
• cystatin 6
• Cystatin E/M
• cystatin M
• cystatin M/E
• Cystatin-6
• cystatin-E
• cystatin-M
• cysteine proteinase inhibitor

Background

Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily (1, 2). It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning (1, 2). However, its protein was found only in skin and sweat glands by a third group (3). In addition to being a cysteine protease inhibitor, Cystatin E/M is also a substrate for transglutaminases (3). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the Cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age (4). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (5).

1. Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
2. Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
3. Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.
4. Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
5. Hong, J. et al. (2002) J. Neurochem. 81:922.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cystatin E/M/CST6 Antibody (MAB1286)(1)

Publication using MAB1286

• Gerdes MJ, Myakishev M, Frost NA, Rishi V, Moitra J, Acharya A, Levy MR, Park SW, Glick A, Yuspa SH, Vinson C Activator protein-1 activity regulates epithelial tumor cell identity. Cancer Res., 2006-08-01;66(15):7578-88. 2006-08-01 [PMID: 16885357] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CST6
• Uniprot:
  • Q15828()