Cystatin E/M/CST6 Antibody (211515) [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Clone: 211515
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 488

Cystatin E/M/CST6 Antibody (211515) [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects human Cystatin E/M in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cystatins A, B, C, S, SA, SN, rhFetuin A, B, rhHRGP, or rhKininogen is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin E/M/CST6 Antibody (211515) [Alexa Fluor™ Plus 488]

• CST6
• cystatin 6
• Cystatin E/M
• cystatin M
• cystatin M/E
• Cystatin-6
• cystatin-E
• cystatin-M
• cysteine proteinase inhibitor

Background

Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily (1, 2). It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning (1, 2). However, its protein was found only in skin and sweat glands by a third group (3). In addition to being a cysteine protease inhibitor, Cystatin E/M is also a substrate for transglutaminases (3). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the Cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age (4). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (5).

1. Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
2. Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
3. Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.
4. Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
5. Hong, J. et al. (2002) J. Neurochem. 81:922.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.