Human Cystatin C ELISA Kit (Colorimetric)

Product Discontinued

Human Cystatin C ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. CST3 (Human ) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human CST3.
• Standard Curve Range: 0.312 ng/mL to 20 ng/mL
• Sensitivity: <10 pg/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CST3

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Cystatin C ELISA Kit (Colorimetric)

• ARMD11
• bA218C14.4 (cystatin C)
• CST3
• cystatin 3
• cystatin C (amyloid angiopathy and cerebral hemorrhage)
• Cystatin C
• cystatin-3
• cystatin-C
• Gamma-trace
• MGC117328
• Neuroendocrine basic polypeptide
• Post-gamma-globulin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Cystatin C ELISA Kit (KA2107). (Showing 1 - 2 of 2 FAQ).

1. I want to carry out a research using cystatin c to determine GFR in sickle cell anemia patients. Can pin prick sample be used? What is the procedure to carry out the test?
   • The cystatin C ELISA kits we sell are manufactured by two companies, Abnova and Biosensis, and distributed by us here at Novus Biologicals. I contacted both companies to ask them your question. I have now heard back from Biosensis, who produce the product catalog # BEK-2145-2P. Their answer was as follows: We haven't used our kit with such samples; however, a recent paper suggests that it could be possible. The paper used a different method than traditional ELISA, but if the samples were allowed to clot or collected in citrated tubes it certainly seems possible to use such samples, but the customer will have to investigate on their own the true effectiveness compared to traditional blood sample collection, for which the kit is tested. Here is a link to the paper examining capillary tube collection (https://academic.oup.com/clinchem/article/51/5/903/5629843) These people even have a patent on using the technology for the measure of cystatin C for human samples (http://helierscientific.co.uk/renalfunction.html) so that too might be an issue for your customer. Finally, this paper too probably should be reviewed by your customer; it is more modern and discusses a number of approaches and issues (https://journals.lww.com/cjasn/pages/articleviewer.aspx?year=2009&issue=11000&article=00029&type=Fulltext) Just to let you know that I have now heard from Abnova, the manufacturer of our cystatin C ELISA kits product catalog # KA0022 (protocol: http://www.abnova.com/protocol_pdf/KA0022.pdf) and catalog # KA2107 (protocol: http://www.abnova.com/protocol_pdf/KA2107.pdf). Their response was simply that neither of the kits have been tested on pin-prick blood samples, and so they are not sure whether these kits are suitable or not.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CST3