Cystatin A Overexpression Lysate

Images

 
Western Blot: Cystatin A Overexpression Lysate (Adult Normal) [NBL1-09553] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cystatin A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Cystatin A Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203115 and based on accession number NM_005213. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cystatin A (stefin A) (CSTA), mRNA.
Gene
CSTA

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
10.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cystatin A Overexpression Lysate

  • AREI
  • CSTA
  • cystatin A (stefin A)
  • Cystatin A
  • cystatin AS
  • cystatin-A
  • Stefin A
  • STF1
  • STF1Cystatin-AS
  • STFA
  • STFAStefin-A

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Cystatin A Lysate (NBL1-09553) (0)

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Video Protocols

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Other Available Formats

NBL1-09553

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Bioinformatics

Gene Symbol CSTA