CYRPTIC/CFC1 Antibody (219708)

• Reactivity: Hu
• Applications: WB
• Clone: 219708
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CYRPTIC/CFC1 Antibody (219708) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cryptic
  Tyr26-Gly169
  Accession # P0CG36
• Specificity: Detects human Cryptic in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse Cryptic is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFC1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CYRPTIC/CFC1 Antibody (219708)

• CFC1
• CFC-1
• CFC1B
• cripto, FRL-1, cryptic family 1
• Cryptic family protein 1
• cryptic protein
• Cryptic
• CRYPTICFLJ77897
• HTX2MGC133213

Background

Cryptic, also known at CFC-1, was originally identified as a secreted protein that is specifically expressed during mesoderm differentiation (1). Cryptic, along with Cripto, Xenopus FRL-1 and zebrafish OEP (one-eyed pinhead) define the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins that function in cancer and various developmental processes (2, 3). Overall sequence identity between members of the family is low, but they do share distinct domains: a variant EGF-like motif, a novel conserved cysteine-rich domain (called CFC domain), and a C-terminal hydrophobic region (2). Most EGF-CFC members have a glycosyl-phosphatidylinositol (GPI) anchoring site at the C-terminus and exist as extracellular membrane-anchored proteins. However, naturally-occurring soluble isoforms also exist. Human Cryptic shares 55% and 25% amino acid idenitity with mouse Cryptic and human Cripto, respectively. Despite weak conservation in amino acid identity, EGF-CFC family members appear to function similarly in assays for phenotypic rescue of zebrafish oep mutants (2). Cryptic is expressed during gastrulation in the mesoderm and later in the neuroectoderm, marking the prospective floor plate of the neural tube (1). Genetic evidence from mice and humans points to a role for Cryptic in determining left-right asymmetry. Mutations in the cryptic gene result in a spectrum of heart, lung and spleen defects, all representing left-right laterality defects (4, 5). These phenotypes resemble some Nodal mutant alleles suggesting that Cryptic, like Cripto, acts as an essential cofactor for Nodal signaling (1, 3). Studies have shown that other TGF-beta superfamily members involved in mesoderm induction and left-right patterning, Vg1 and GDF-1, also require EGF-CFC cofactors. Cryptic binds to GDF-1 leading to an Act RIIB-ALK4-Cryptic-GDF-1 complex for signaling (6).

1. Shen, M. et al. (1997) Development 124:429. 
2. Shen, M. and A. Schier (2000) Trends Genet. 16:303.
3. Rosa, F.M. (2002) Science's STKE http://stke.sciencemag.org/.
4. Gaio, U. et al. (1999) Curr. Biol. 9:1339.
5. Bamford, R. et al. (2000) Nature Genet. 26:365.
6. Cheng, S. et al. (2003) Genes & Dev. 17:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFC1
• Uniprot:
  • P0CG36()