Cyr61/CCN1 Antibody (365108)

• Reactivity: Hu
• Applications: WB
• Clone: 365108
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cyr61/CCN1 Antibody (365108) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cyr61
  Ala22-Asp381
  Accession # O00622
• Specificity: Detects human Cyr61/CCN1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYR61
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyr61/CCN1 Antibody (365108)

• CCN family member 1
• CCN1
• CCN1IGF-binding protein 10
• Cyr61
• Cysteine-rich angiogenic inducer 61
• cysteine-rich, angiogenic inducer, 61
• GIG1
• GIG1cysteine-rich heparin-binding protein 61
• IBP-10
• IGFBP-10
• IGFBP10cysteine-rich, anigogenic inducer, 61
• Insulin-like growth factor-binding protein 10
• protein CYR61
• Protein GIG1

Background

Cyr61, also known as CCN1, is a 40 - 45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4 - 6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is upregulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8 - 12). In contrast, when downregulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also upregulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).

1. Chen, Y. and X.Y. Du (2007) J. Cell. Biochem. 100:1337.
2. Jay, P. et al. (1997) Oncogene 14:1753.
3. Feng, P. et al. (2008) Int. J. Biochem. Cell Biol. 40:98.
4. Chen, N. et al. (2000) J. Biol. Chem. 275:24953.
5. Leu, S-J. et al. (2002) J. Biol. Chem. 277:46248.
6. Schutze, N. et al. (2007) BMC Cell Biol. 8:45.
7. Pendurthi, U.R. et al. (2005) Cancer Res. 65:9705.
8. O'Kelly, J. et al. (2008) Int. J. Oncol. 33:59.
9. Chintalapudi, M.R. et al. (2008) Carcinogenesis 29:696.
10. Xie, D. et al. (2004) Cancer Res. 64:1987.
11. Lin, M-T. et al. (2007) J. Biol. Chem. 282:34594.
12. Monnier, Y. et al. (2008) Cancer Res. 68:7323.
13. Tong, X. et al. (2001) J. Biol. Chem. 276:47709.
14. Chien, W. et al. (2004) J. Biol. Chem. 279:53087.
15. Hadjiargyrou, M. et al. (2000) J. Bone Miner. Res.15:1014.
16. Chen, C-C. et al. (2001) J. Biol. Chem. 276:47329.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyr61/CCN1 Antibody (MAB4055)(5)

Publications using MAB4055

• N McNamee, LR de la Fuen, MJ Santos-Mar, L O'Driscoll Proteomics profiling identifies extracellular vesicles' cargo associated with tumour cell induced platelet aggregation BMC Cancer, 2022-09-29;22(1):1023. 2022-09-29 [PMID: 36171564] (Western Blot, Human)
• T Song, D Spillmann Transcriptomic analysis reveals cell apoptotic signature modified by heparanase in melanoma cells J. Cell. Mol. Med., 2019-05-02;0(0):. 2019-05-02 [PMID: 31044520] (Western Blot, Human)
• Gao W, Cai L, Xu X, Fan J, Xue X, Yan X, Qu Q, Wang X, Zhang C, Wu G Anti-CTGF single-chain variable fragment dimers inhibit human airway smooth muscle (ASM) cell proliferation by down-regulating p-Akt and p-mTOR levels. PLoS ONE, 2014-12-05;9(12):e113980. 2014-12-05 [PMID: 25478966] (ELISA Development, Human)
• Jeansonne D, Pacifici M, Lassak A et al. Differential Effects of MicroRNAs on Glioblastoma Growth and Migration. Genes (Basel) 4/8/2014 [PMID: 24705102]
• Monnier Y, Farmer P, Bieler G, Imaizumi N, Sengstag T, Alghisi GC, Stehle JC, Ciarloni L, Andrejevic-Blant S, Moeckli R, Mirimanoff RO, Goodman SL, Delorenzi M, Ruegg C CYR61 and alphaVbeta5 integrin cooperate to promote invasion and metastasis of tumors growing in preirradiated stroma. Cancer Res., 2008-09-15;68(18):7323-31. 2008-09-15 [PMID: 18794119] (Western Blot, Human)

Review for Cyr61/CCN1 Antibody (MAB4055) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 04/12/2022

Summary

• Application: Western Blot
• Sample Tested: Saos-2 human osteosarcoma cell line
• Species: Human
• Lot: 365108

Bioinformatics

• Gene Symbol: CYR61
• Uniprot:
  • O00622(Human)