CYP8B1 Antibody

Images

 
Western Blot: CYP8B1 Antibody [NBP2-29939] - Western blot analysis of (C-term) (NBP2-29939 ) in K562 cell line lysates (35ug/lane). CYP8B1 (arrow) was detected using the purified Pab.
Immunohistochemistry-Paraffin: CYP8B1 Antibody [NBP2-29939] - Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB ...read more
Flow Cytometry: CYP8B1 Antibody [NBP2-29939] - Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

CYP8B1 Antibody Summary

Immunogen
This CYP8B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 471-501 amino acids from the C-terminal region of human CYP8B1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP8B1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10 - 1:50
  • Immunohistochemistry-Paraffin 1:10 - 1:50
Theoretical MW
58.068 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
2 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for CYP8B1 Antibody

  • CP8B
  • CYP127 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
  • CYPVIIIB1
  • Cytochrome P450 8B1
  • cytochrome P450, family 8, subfamily B, polypeptide 1
  • cytochrome P450, subfamily VIIIB (sterol 12-alpha-hydroxylase), polypeptide 1,7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
  • EC 1.14.13.95
  • FLJ17826,7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
  • Sterol 12-alpha-hydroxylase

Background

CYP8B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Array NBP2-29939

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Diseases for CYP8B1 Antibody (NBP2-29939)

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Bioinformatics

Gene Symbol CYP8B1
Uniprot