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CYP51A1 Antibody

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Western Blot: CYP51A1 Antibody [NBP2-16071] - Sample (30 ug of whole cell lysate) A: THP-1 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: CYP51A1 Antibody [NBP2-16071] - Sample (30 ug of whole cell lysate) A: THP-1 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Bv, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CYP51A1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CYP51A1. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane; Microsome membrane
Predicted Species
Primate (98%), Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP51A1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (98%), Xenopus laevis (84%), Chicken (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for CYP51A1 Antibody

  • CP51
  • CYP51Cytochrome P450LI
  • CYPL1
  • CYPLI
  • Cytochrome P450 51A1
  • cytochrome P450, family 51, subfamily A, polypeptide 1
  • Cytochrome P450-14DM
  • EC 1.14.13.70
  • lanosterol 14-alpha demethylase
  • lanosterol 14-alpha-demethylase
  • LDMCytochrome P45014DM
  • P450-14DM
  • P450L1,51 (lanosterol 14-alpha-demethylase)
  • Sterol 14-alpha demethylase

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.
Immunohistochemistry: MBD2 Antibody [NB100-81657] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MBD2 used at a dilution of 1:200 (1ug/ml). Detection: DAB
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Insulin Antibody
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SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]
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Mas Antibody
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Immunocytochemistry/Immunofluorescence: Mas Antibody [NBP1-78444] - MAS1 antibody was tested at 1:25 in HepG2 cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunohistochemistry: Mas Antibody [NBP1-78444] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.
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Glut4 Antibody
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Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. This image was submitted via customer review.
Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney
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CYP7A1 Antibody
(946902) [Unconjugate
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Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Western blot analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Immunofluorescence analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.
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POR/Cytochrome
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PRB1
Overexpression Lysate
NBP2-04611
Western Blot: PRB1 Overexpression Lysate (Adult Normal) [NBP2-04611] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRB1.
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Western Blot: CREM Antibody [NBP1-81760] - Analysis in human cell lines PC-3 and Caco-2 using Anti-CREM antibody. Corresponding CREM RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
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LDL R Antibody (C7)
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Western Blot: LDL R Antibody (C7) [NBP1-78159] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-LDL receptor in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2.
Immunohistochemistry: LDL R Antibody (C7) [NBP1-78159] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin counterstaining.
(1 Review)
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Western Blot: Glut1 Antibody [NB110-39113] - Analysis of GLUT1 on A. mouse kidney membrane protein and B. rat kidney membrane protein.
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).
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Western Blot: PLOD1 Antibody [NBP2-38770] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
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Bioinformatics

Gene Symbol CYP51A1
Uniprot