Cyclophilin-F Antibody

Images

 
Western Blot: Cyclophilin-F Antibody [NBP2-15079] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PPIF antibody [N1C3] diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Cyclophilin-F Antibody [NBP2-15079] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Cyclophilin-F Antibody [NBP2-15079] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Western Blot: Cyclophilin-F Antibody [NBP2-15079] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Cyclophilin-F Antibody [NBP2-15079] - ouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PPIF antibody [N1C3] diluted at 1:5000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cyclophilin-F Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cyclophilin F. The exact sequence is proprietary.
Predicted Species
Bovine (90%), Rhesus Macaque (99%), Chicken (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPIF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Cyclophilin-F Knockout 293T Cell Lysate
Publications
Read Publication using NBP2-15079.

Reactivity Notes

Xenopus laevis (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Cyclophilin-F Antibody

  • Cyclophilin F
  • CYP3FLJ90798
  • Cyp-D
  • hCyP3
  • MGC117207
  • mitochondrial
  • peptidyl-prolyl cis-trans isomerase, mitochondrial
  • peptidylprolyl isomerase F (cyclophilin F)
  • peptidylprolyl isomerase F

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Cyclophilin-F Antibody (NBP2-15079)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PPIF
Uniprot