Cyclophilin-F Antibody

Western Blot: Cyclophilin-F Antibody [NBP2-15079] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunocytochemistry/Immunofluorescence: Cyclophilin-F Antibody [NBP2-15079] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Cyclophilin-F Antibody [NBP2-15079] - Human colon carcinoma, using PPIF antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Cyclophilin-F Antibody [NBP2-15079] - ouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PPIF antibody [N1C3] diluted at 1:5000.

Western Blot: Cyclophilin-F Antibody [NBP2-15079] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PPIF antibody [N1C3] diluted at 1:500.

Knockout Validated: Cyclophilin-F Antibody [NBP2-15079] - Wild-type (WT) and Cyclophilin F knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin-F antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cyclophilin-F Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cyclophilin F. The exact sequence is proprietary.
• Predicted Species: Bovine (90%), Rhesus Macaque (99%), Chicken (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPIF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:10000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Cyclophilin-F Antibody

• Cyclophilin F
• CYP3FLJ90798
• Cyp-D
• hCyP3
• MGC117207
• mitochondrial
• peptidyl-prolyl cis-trans isomerase, mitochondrial
• peptidylprolyl isomerase F (cyclophilin F)
• peptidylprolyl isomerase F

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Cyclophilin-F Antibody (NBP2-15079)(1)

Publication using NBP2-15079

• Mulay SR, Honarpisheh MM, Foresto-Neto O et al. Mitochondria Permeability Transition versus Necroptosis in Oxalate-Induced AKI J. Am. Soc. Nephrol. 2019-07-11 [PMID: 31296606]

Bioinformatics

• Gene Symbol: PPIF
• Uniprot:
  • P30405(Human)