Cyclin L2 Antibody

Western Blot: Cyclin L2 Antibody [NB100-87009] - Whole cell lysate (50 ug) from HEK293T, K-562, HeLa, U2OS, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Cyclin L2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: Cyclin L2 Antibody [NB100-87009] - Detection of human Cyclin L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Cyclin L2 antibody NB100-87009 (lot 2) used for IP at 6 ug per reaction. Cyclin L2 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-Cyclin L2 antibody from Company B. For blotting immunoprecipitated Cyclin L2, NB100-87009 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, IP, WB, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cyclin L2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 470 and 520 of human cyclin L2 using the numbering given in entry NP_112199.2 (GeneID 81669).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCNL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunoprecipitation 2-10 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
• Application Notes: Knockdown validation (PMID: 31852782). Use in immunoblotting reported in scientific literature (PMID:31852782).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cyclin L2 Antibody

• ania-6b
• CCNM
• cyclin L2
• cyclin M
• cyclin-L2
• DKFZp761A1210
• DKFZp762O195
• HCLA-ISO
• HLA-ISO
• Paneth cell-enhanced expression protein
• PCEE
• SB138

Background

Cyclin L2 is a member of the cyclin family of regulatory proteins that associate with and activate cyclin-dependent kinases (CDKs) to regulate cell cycle progression and transcription. The hallmark characteristic of cyclins is the periodicity of their expression and activity that is regulated transcriptionally and post-transcriptionally via phosphorylation and ubiquitination. Cyclin L2 is part of a group of cyclins that are involved in transcriptional regulation at the level of RNA transcription, initiation, elongation, and RNA processing. The transcriptional regulating cyclins include cyclin C, H, K, L1, L2, T1, and T2 which associate with CDK7, -8, -9, -10 and -12. Cyclin L2 associates with CDK11 and RNA polymerase II and co-localizes with splicing factors and is implicated to play a role in pre-mRNA splicing. Alternate names for cyclin L2 include cyclin M, paneth cell-enhanced expression protein, CCNM, and CCNL2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclin L2 Antibody (NB100-87009)(3)

Publications using NB100-87009

• Parrish PCR, Thomas JD, Gabel AM Et al. Discovery of synthetic lethal and tumor suppressor paralog pairs in the human genome Cell reports 2021-08-31 [PMID: 34469736] (WB, Human)
• Kisaka JK, Ratner L, Kyei GB The dual specificity kinase DYRK1A modulates the levels of cyclin L2 to control HIV replication in macrophages J. Virol. 2019-12-18 [PMID: 31852782] (IP, IB, KD, Human)
• Kyei GB, Cheng X, Ramani R, Ratner L. Cyclin L2 Is a Critical HIV Dependency Factor in Macrophages that Controls SAMHD1 Abundance. Cell Host Microbe. 2014-12-17 [PMID: 25532805] (WB, Human)

Bioinformatics

• Gene Symbol: CCNL2
• Entrez:
  • 81669(Human)
• Uniprot:
  • Q96S94(Human)