CrkRS Antibody

Western Blot: CrkRS Antibody [NB100-87011] - Detection of Human and Mouse CrkRS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: CrkRS Antibody [NB100-87011] - Detection of human CrkRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for IP at 6 ug per reaction. For blotting immunoprecipitated CrkRS, NB100-87011 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CrkRS Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human cell division cycle 2 (CDC2)-related kinase arginine/serine-rich using the numbering given in entry NP_057591.2 (GeneID 51755).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in WB reported in scientific literature ( PMID 24478330).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CrkRS Antibody

• Cdc2-related kinase, arginine/serine-richCrkRS
• Cell division cycle 2-related protein kinase 7
• Cell division protein kinase 12
• CRK7CDC2-related protein kinase 7
• CRKR
• CRKRS
• cyclin-dependent kinase 12
• EC 2.7.11
• EC 2.7.11.23
• hCDK12
• KIAA0904EC 2.7.11.22

Background

CrkRS (Cdc2-related kinase, arginine/serine-rich) is a Cdc-2 related kinase that contains an arginine- and serine-rich (SR) domain and has recently been reported to interact with cyclin L1 and L2. Since it has been discovered that CrkRs pairs with a cyclin partner, it has also been given the name CDK12. CrkRs localizes to nuclear speckles and immunoprecipitates with RNA polymerse II and the SF2/ASF splicing factor and is proposed to play a role in the regulation of alternative splicing. Alternate names for CrkRS include cell division cycle 2-related protein kinase 7, CDC2-related protein kinase 7, CRKRS, CRK7, and KIAA0904.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CrkRS Antibody (NB100-87011)(6)

Publications using NB100-87011

• Guiro J, Fagbemi M, Tellier M et al. CAPTURE of the Human U2 snRNA Genes Expands the Repertoire of Associated Factors Biomolecules 2022-05-14 [PMID: 35625631] (WB)
• Chai Q, Li S, Collins MK et al. HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity Cell Reports 2021-08-10 [PMID: 34380030]
• Quereda V, Bayle S, Vena F et al. Therapeutic Targeting of CDK12/CDK13 in Triple-Negative Breast Cancer Cancer Cell 2019-10-08 [PMID: 31668947]
• Eifler Tt, Shao W, Bartholomeeusen K et al. CDK12 increases 3' end processing of growth factor-induced c-FOS transcripts. Mol. Cell. Biol. 2014-11-10 [PMID: 25384976]
• Davidson L, Muniz L, West S. 3' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells. Genes Dev. 2014-02-17 [PMID: 24478330] (WB, Human)
• Friedrich MS Understanding and Exploiting MYCN Addiction in High-Risk Neuroblastoma Using the Example of CDK13 Thesis 2021-01-01 (WB, Human)

Bioinformatics

• Gene Symbol: CDK12
• Entrez:
  • 51755(Human)
• Uniprot:
  • Q9NYV4(Human)