Cyclin C Antibody

Western Blot: Cyclin C Antibody [NB120-2950] - WB analysis using a Cyclin C polyclonal antibody in HL-60 cell lysates (35 ug per lane).

Product Discontinued

Cyclin C Antibody Summary

• Immunogen: A synthetic peptide corresponding to aa 290-303 (Cys-QGPNGSQNSSYSQS) from the C-terminal of human cyclin C
• Localization: Nuclear
• Specificity: Cyclin C
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCNC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 10 ug/mg
  • Knockdown Validated
  • Western Blot 5 - 10 ug/mL
• Application Notes: Use in KD reported in secitific publication PMID: 32560467
• Control:

  HeLa Whole Cell Lysate
  Cyclin C Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cyclin C Antibody

• CycC
• cyclin C
• cyclin-C
• hSRB11
• SRB11 homolog

Background

Cyclin C was originally identified by a genetic screen for human and Drosophila cDNAs that complement a triple knock-out of the CLN genes in Saccharomyces cerevisiae. Cyclin C is a nuclear protein present in a multiprotein complex. It interacts both in vitro and in vivo with cdk8, a novel protein-kinase of the cdk family. It is suggested that cyclin C and cdk8 control RNA polymerase II function. Human cdk8-cyclin C might be functionally associated with the mammalian transcription apparatus, perhaps involved in relaying growth-regulatory signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclin C Antibody (NB120-2950)(1)

Publication using NB120-2950

• Butler M, Chotiwan N, Brewster CD et al. Cyclin-Dependent Kinases 8 and 19 Regulate Host Cell Metabolism during Dengue Virus Serotype 2 Infection Viruses 2020-06-17 [PMID: 32560467] (KD, WB, Human)

Bioinformatics

• Gene Symbol: CCNC
• Entrez:
  • 892(Human)
  • 51813(Mouse)
  • 114839(Rat)
• Uniprot:
  • P24863(Human)