Cyclin B2 Antibody (SD2045)

Western Blot: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Western blot analysis of Cyclin B2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti

Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Staining Cyclin B2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Analysis of paraffin-embedded mouse testis tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.

Western Blot: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: K562

Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Staining Cyclin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Analysis of paraffin-embedded mouse colon tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.

Product Discontinued

Cyclin B2 Antibody (SD2045) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Cyclin B2 aa 1-102 / 398. (SwissProt: O95067 Human; SwissProt: P30276 Mouse; Entrez Gene:363088 Rat)
• Localization: Centrosome, microtubule cytoskeleton, cytosol, nucleus, cyclin-dependent protein kinase holoenzyme complex, cytoplasm, host cell nucleus, membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCNB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cyclin B2 Antibody (SD2045)

• CCNB2
• Cyclin B2
• G2/mitotic-specific cyclin-B2
• HsT17299

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCNB2