Cyclin B2 Antibody (SD2045)

Images

 
Western Blot: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Western blot analysis of Cyclin B2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Staining Cyclin B2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Analysis of paraffin-embedded mouse testis tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.
Western Blot: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: K562
Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Staining Cyclin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: Cyclin B2 Antibody (SD2045) [NBP2-67749] - Analysis of paraffin-embedded mouse colon tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
SD2045
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cyclin B2 Antibody (SD2045) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within human Cyclin B2 aa 1-100. (SwissProt: O95067 Human ;SwissProt: P30276 Mouse ;EntrezGene:363088 Rat)
Localization
Centrosome, microtubule cytoskeleton, cytosol, nucleus, cyclin-dependent protein kinase holoenzyme complex, cytoplasm, host cell nucleus, membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CCNB2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Cyclin B2 Antibody (SD2045)

  • CCNB2
  • Cyclin B2
  • G2/mitotic-specific cyclin-B2
  • HsT17299

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CCNB2