Cyclin A1 Antibody

Western Blot: Cyclin A1 Antibody [NBP1-02902] - Cyclin A1 western with U2OS cells. Image from verified customer review.

Immunohistochemistry-Paraffin: Cyclin A1 Antibody [NBP1-02902] - Human testis after heat-induced antigen retrieval. Antibody dilution 1:500.

Product Discontinued

Cyclin A1 Antibody Summary

• Immunogen: Full length recombinant protein
• Specificity: Antiserum will specifically react with a 60 kDa Cyclin A11 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin A11 from other species may also occur.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCNA1
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000
• Application Notes: IHC: NBP1-02902 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Delipidated and defibrinated serum
• Preservative: 0.01% Sodium Azide
• Concentration: 80 mg/ml
• Purity: Delipidation and Defibrination

Alternate Names for Cyclin A1 Antibody

• CCNA1
• Cyclin A1
• cyclin-A1

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclin A1 Antibody (NBP1-02902)(1)

Publication using NBP1-02902

• Ochsenreither S, Majeti R, Schmitt T et al. Cyclin-A1 represents a new immunogenic targetable antigen expressed in acute myeloid leukemia stem cells with characteristics of a cancer-testis antigen Blood 2012-06-07 [PMID: 22529286]

Review for Cyclin A1 Antibody (NBP1-02902) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/19/2014

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate from HeLa cells
• Species: Human

FAQs for Cyclin A1 Antibody (NBP1-02902). (Showing 1 - 1 of 1 FAQ).

1. I'd like to know if this antibody cross reacts with mouse, and if not/yes how this was tested?
   • This antibody has not been tested in mouse however I have checked the % homology between the mouse and human protein and this is 84%. Our lab recommends use if the % homology is 85% or above. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

Bioinformatics

• Gene Symbol: CCNA1
• Entrez:
  • 8900(Human)
• Uniprot:
  • P78396()