Cyclin A1 Antibody

Images

 
Western Blot: Cyclin A1 Antibody [NBP1-02902] - Cyclin A1 western with U2OS cells. Image from verified customer review.
Immunohistochemistry-Paraffin: Cyclin A1 Antibody [NBP1-02902] - Human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
80 mg/ml

Order Details

Cyclin A1 Antibody Summary

Immunogen
Full length recombinant protein
Specificity
Full length synthetic polypeptide corresponding to the human gene sequence.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCNA1
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:10-1:500
Reviewed Applications
Read 1 Review rated 3
using
NBP1-02902 in the following applications:

Publications
Read Publication using NBP1-02902.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Delipidated and defibrinated serum
Preservative
0.01% Sodium Azide
Concentration
80 mg/ml
Purity
Delipidation and Defibrination

Alternate Names for Cyclin A1 Antibody

  • CCNA1
  • Cyclin A1
  • cyclin-A1

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-02902
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for Cyclin A1 Antibody (NBP1-02902)(1)

Review for Cyclin A1 Antibody (NBP1-02902) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-02902:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Cyclin A1 NBP1-02902
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reviewed by:
Anonymous
WB Human 09/19/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from HeLa cells
SpeciesHuman
CommentsThe antibody gives a strong band for predicted size along with a few other faint bands.

Blocking

Blocking Details5% NFDM in PBS+0.1% Triton

Primary Anitbody

Dilution Ratio700 times diluted in PBS, incubated for 2 hours at room temperature

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody (Cell Signaling)
Secondary Manufacturer Cat#7074
Secondary Concentration20,000

Details

Detection NotesCaptured using X-rays (5 sec exposure) with Phototope-HRP Western Blot Detection System

Comments

CommentsThe antibody gives a strong band for predicted size along with a few other faint bands.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cyclin A1 Antibody (NBP1-02902). (Showing 1 - 1 of 1 FAQ).

  1. I'd like to know if this antibody cross reacts with mouse, and if not/yes how this was tested?
    • This antibody has not been tested in mouse however I have checked the % homology between the mouse and human protein and this is 84%. Our lab recommends use if the % homology is 85% or above. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Cyclin A1 Antibody (NBP1-02902)

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Calculators

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Recent Reviews

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Anonymous
09/19/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CCNA1
Entrez
Uniprot