CYB5R3 Antibody

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Western Blot: CYB5R3 Antibody [NBP1-51984] - Analysis of CYB5R3 in Mouse Liver lysate (RIPA buffer, 35ug total protein per lane) using NBP1-51984 at 0.1ug/ml. Primary incubated for 1 hour. Detected by western blot using ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

CYB5R3 Antibody Summary

Immunogen
Peptide with sequence PNLERVGHPKERC corresponding to C-Terminus according to Uniprot Mouse NP_084063.1.
Epitope
PNLERVGHPKERC
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CYB5R3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 35 kDa band observed in mouse liver lysates as well as in mouse and rat brain lysates (calculated MW of 34.1 kDa band according to mouse NP_084063.1).
Publications
Read Publication using NBP1-51984.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CYB5R3 Antibody

  • B5RNADH-cytochrome b5 reductase 3
  • cytochrome b5 reductase 3
  • Cytochrome b5 reductase
  • DIA1NADH-cytochrome b5 reductase 3 membrane-bound form
  • diaphorase (NADH) (cytochrome b-5 reductase)
  • diaphorase-1
  • EC 1.6.2.2
  • NADH-cytochrome b5 reductase 3 soluble form

Background

Two forms of NADH-cytochrome b5 reductase are known, a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The former exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains. The soluble form has only the catalytic domain. This gene encodes both forms of the enzyme which arise from tissue-specific alternative transcripts that differ in the first exon. Mutations in this gene cause methemoglobinemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CYB5R3 Antibody (NBP1-51984)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CYB5R3
Entrez
Uniprot