CXCR7/RDC-1 Antibody

Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CXCR7/RDC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4227) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CXCR7/RDC‑1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Undifferentiated mouse neural progenitor cells were stained with Sheep Anti-Mouse CXCR7/RDC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4227, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR7/RDC-1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CXCR7 extracellular N-terminus and loops
  Met1-Leu47, Ser103-Lys118, Lys184-Glu213, and Leu274-Ala296
  Accession # P56485
• Specificity: Detects CXCR7/RDC‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse CXCR5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CXCR7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR7/RDC-1 Antibody

• ACKR3
• chemokine (C-X-C motif) receptor 7
• Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
• CMKOR1
• CXCR7
• CXC-R7
• CXCR-7
• G protein-coupled receptor
• GPR159
• GPR159G-protein coupled receptor 159
• RDC-1
• RDC1G-protein coupled receptor RDC1 homolog

Background

CXCR7 (CXC chemokine receptor 7; also GPRN1, RDC1 and chemokine orphan receptor 1) is a 60 kDa member of the G-protein coupled receptor 1 family. It is expressed on multiple cell types, including neurons, T cells, NK cells, neutrophils, B cells plus angiogenic endothelial cells. CXCR7 forms both homodimers and heterodimers with CXCR4. It selectively binds I-TAC and SDF1, and appears to involve beta -arrestin2 during signaling. Notably, a CXCR7:CXCR4 heterodimer shows increased responsiveness to SDF1, and I-TAC may actually block some SDF1-mediated migration activity. Mouse CXCR7 is a 7-transmembrane glycoprotein that is 362 amino acids (aa) in length. It contains a 47 aa N-terminal extracellular region plus a 43 aa C-terminal cytoplasmic domain. Over aa 1‑47, 103‑118, 184‑213 and 274‑296 collectively, mouse CXCR7 shares 97% and 91% aa identity with rat and human CXCR7, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR7/RDC-1 Antibody (AF4227)(2)

Publications using AF4227

• Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury. PLoS ONE, 2012;7(4):e34608. 2012 [PMID: 22511954] (Neutralization, Mouse)
• Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs. Biochem. Biophys. Res. Commun., 2010;401(4):509-15. 2010 [PMID: 20869949] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CXCR7
• Uniprot:
  • P56485()