CXCR6 Antibody (1010910) [Unconjugated]

Human CD3+ peripheral blood mononuclear cells (PBMCs) cultured with plate bound anti-Human CD3 and CD28 Antibodies, Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 μg/mL) and 10 ng/mL Recombinant Human IL‑12 (Catalog # 219-IL) for 5 days to induce Th1 cell development were stained with (A) Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB6991) or (B) Isotype control antibody (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 1010910
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCR6 Antibody (1010910) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human CXCR6
  Met1-Leu342
  Accession # O00574
• Specificity: Detects human CXCR6 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR6 Antibody (1010910) [Unconjugated]

• Bonzo
• BONZOG-protein coupled receptor bonzo
• CD186 antigen
• CD186
• CDw186
• chemokine (C-X-C motif) receptor 6
• C-X-C chemokine receptor type 6
• CXCR6
• CXC-R6
• CXCR-6
• G protein-coupled receptor
• STRL33
• STRL33G-protein coupled receptor STRL33
• TYMSTRCD186

Background

CXCR6, also known as BONZO, STRL33, TYMSTR, is the receptor for the C-X-C chemokine CXCL16. CXCR6 is a G protein coupled protein receptor (GPCR) expressed on activated T cells and tumor-infiltrating lymphocytes, and it has been identified as an entry co-receptor used by HIV-1 and SIV to enter target cells, in conjunction with CD4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O00574()